Summary
Starting nucleotide sequences of RNA synthesized using T7 DNA and E. coli RNA polymerase holoenzyme were determined. A ratio of the number of RNA started with ATP to that with GTP was 2.4 in the presence of 200 mM KCl, when more than 90% of the former had a unique sequence, pppApUpCpGp--and most of the latter had pppGpPyp--. On the other hand, at low KCl concentration, other sequences were observed.
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Communicated by H. Ozeki
Number II of this series was published in Molec. gen. Genet. 118, 23 (1972).
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Iida, Y., Matsukage, A. The effect of KCl concentration of the transcription by E. coli RNA polymerase. Molec. Gen. Genet. 129, 27–35 (1974). https://doi.org/10.1007/BF00269263
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DOI: https://doi.org/10.1007/BF00269263