Skip to main content
SpringerLink
Log in

Studies on endosperm culture of Annona squamosa Linn

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Mature endosperm tissue excised from germinated seeds (2–4 days after radicle emergence) of Annona squamosa grew and proliferated on White's basal medium supplemented with two cytokinins, an auxin and gibberellic acid. The callus obtained could be periodically subcultured. Shoot differentiation and root induction were obtained from callus on media of different compositions. Analyses of the root and young leaf tips showed triploid number of chromosomes (3n=21).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

Kin:

Kinetin

BAP:

6-Benzylaminopurine

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

NAA:

∝naphthalene abetic acid

GA3 :

Gibberellic acid

References

  1. Bhojwani SS (1966) Phytomorphology 16:349–353.

    Google Scholar 

  2. Bhojwani SS, Bhatnagar SP (1978) Embryology of Angiosperms, Vikas Publishing House, Pvt. Ltd. p. 153.

  3. Bhojwani SS, Johri BM (1970) Z, Pflanzenphysiol 63: 269–275.

    Google Scholar 

  4. Brown DJ, Canvin DT, Zilkey BF (1970) Can J. Bot. 48: 2323–2331.

    Google Scholar 

  5. Doig RI, Colborn AJ, Morris G, Laidman DL (1975) J. Exp. Bot. 26:387–398

    Google Scholar 

  6. Johri BM, Srivastava PS, Raste AP (1980) International Review of Cytology Supplement III B, Academic Press, P. 157–182.

  7. LaxmiSita G, RaghavRam NV, Vaidyanathan CS (1980) Plant Sci. Lett. 20:63–69.

    Google Scholar 

  8. Lee J, Chen C (1982) Taiwania 27:86–92.

    Google Scholar 

  9. Nitsch JP (1969) Phytomorphology 19:389–404.

    Google Scholar 

  10. Paleg L (1960) Plant Physiol 35:293–299

    Google Scholar 

  11. Rangaswamy NS, Rao PS (1963) Phytomorphology 13:20–30.

    Google Scholar 

  12. Sharma AK, Sharma A (1980) Chromosome Techniques, Theory and Practice, Third Edition, Butterworths & Co. Ltd.

  13. Srivastava PS (1971) Ph.D. thesis, Univ. of Delhi, Delhi.

  14. Srivastava PS, Johri BM (1974) Beitr. Biol. pflanz. 50: 255–257.

    Google Scholar 

  15. Wang T, Chang C (1978) Proc. Symp. Plant Tissue Culture, Peking, p. 463–468.

  16. White PR (1963) The cultivation of animal and plant cells, Ronald Press Co. N. Y., p. 228–229.

    Google Scholar 

  17. Wrigley A, Lord JM (1977) J. Expt. Bot 28: 345–353.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Biochemical Sciences, National Chemical Laboratory, 411 008, Pune, India

    Sreelata Nair, M. V. Shirgurkar & A. F. Mascarenhas

Authors
  1. Sreelata Nair
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. V. Shirgurkar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. F. Mascarenhas
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by I. K. Vasil

Communication No. 3885

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nair, S., Shirgurkar, M.V. & Mascarenhas, A.F. Studies on endosperm culture of Annona squamosa Linn. Plant Cell Reports 5, 132–135 (1986). https://doi.org/10.1007/BF00269252

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00269252

Keywords

Search

Navigation