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In vitro haploid plantlet induction in Physalis ixocarpa brot. through microspore embryogenesis

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Abstract

Anthers of Physalis ixocarpa Brot. exised from 2–3 mm long flower buds were treated for 2 d at 3°C, and were cultured on the basal medium of NN (1969), supplemented with plant hormones. They formed embryoids from microspores within 6 weeks. Upon transfer to a regeneration medium, embryoids grew into functional plants with the haploid number of chromosomes (n=12).

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Abbreviations

CM:

Coconut-milk

IAA:

indole acetic acid

Kn:

Kinetin

MS:

Murashige and Skoog

NN:

Nitsch and Nitsch

ZEA:

Zeatin

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Author notes

  1. V. A. Bapat

    Present address: Bio-Organic Division, Bhabha Atomic Research Centre, Bombay, India

  2. G. Wenzel

    Present address: Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Resistenzgenetik, D-8059, Grünbach, FRG

Authors and Affiliations

  1. Max-Planck-Institut für Züchtungsforschung (Erwin-Baur-Institut), D-5000, Köln 30, Federal Republic of Germany

    V. A. Bapat & G. Wenzel

Authors
  1. V. A. Bapat
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  2. G. Wenzel
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Bapat, V.A., Wenzel, G. In vitro haploid plantlet induction in Physalis ixocarpa brot. through microspore embryogenesis. Plant Cell Reports 1, 154–156 (1982). https://doi.org/10.1007/BF00269186

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  • DOI: https://doi.org/10.1007/BF00269186

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