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Inactivation of aphidicolin by plant cells

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Abstract

Plant cells are endowed with an aphidicolin inactivating activity. Data on cultured cells show that the rate of inactivation depends on the cell type, Daucus carota cells being the most effective among the other tested materials (Oryza sativa and Nicotiana plumbaginifolia). Also germinating seedling of Haplopappus gracilis and of Citrullus vulgaris inactivate aphidicolin.

Inactivation, which may lead to unexpected results when a prolonged incubation with the drug is required, as in the case of the induction of synchrony of the cell cycle by aphidicolin, can be controlled by appropriately choosing the experimental conditions.

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Abbreviations

2,4-D:

dichlorophenoxyacetic acid

DMSO:

dimethyl sulfoxide

SCV:

ml of sedimented cells in 100 ml of culture

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Authors and Affiliations

  1. Istituto di Genetica Biochimica ed Evoluzionistica, C.N.R., Via S. Epifanio 14, Pavia, Italia

    F. Sala, G. Pedrali-Noy & S. Spadari

  2. Dipartimento di Genetica e Microbiologia, Università di Pavia, Via S. Epifanio 14, Italia

    C. Sala

  3. Dipartimento di Biologia, Università di Milano, Via Celoria, Italia

    M. G. Galli

  4. Gruppo di Biologia D.G. XII, Centro Comune di Ricerca, Ispra, Italia

    E. Nielsen

Authors
  1. F. Sala
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  2. C. Sala
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  3. M. G. Galli
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  4. E. Nielsen
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  5. G. Pedrali-Noy
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  6. S. Spadari
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Sala, F., Sala, C., Galli, M.G. et al. Inactivation of aphidicolin by plant cells. Plant Cell Reports 2, 265–268 (1983). https://doi.org/10.1007/BF00269157

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  • DOI: https://doi.org/10.1007/BF00269157

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