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In vitro plantlet regeneration through asexual embryogenesis in cotyledonary segments of Corylus avellana L.

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Abstract

Cotyledonary nodes of corylus avellana L. excised from young seedlings cultured in K(h) medium for 5 weeks were used to induce embryoid formation. Embryogenesis was achieved in 60% of the explants over two 20-day culture steps in the presence of IBA (5 μM) plus BAP (0.5 μM) and BAP (5 μM) plus IBA (0.5 μM) respectively. Subsequent proliferation was successfully maintained during 5 subcultures on K(h) basal medium and in an unlimited number of subcultures in the presence of BAP (0.5 μM). Plant regeneration (50–55%) was easily achieved by planting into a basal K(h) medium after 10–20 days.

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Abbreviations

BAP:

6-benzvlaminopurine

BM:

basal medium

GA3 :

gibberellic acid

IBA:

indole-3 butyric acid

K(h) medium:

see Cheng 1975

References

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Authors and Affiliations

  1. Departamento de Fisiología Vegetal, Facultad de Biología, Universidad de Oviedo, Oviedo, Spain

    C. Pérez, B. Fernández & R. Rodríguez

Authors
  1. C. Pérez
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  2. B. Fernández
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  3. R. Rodríguez
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Communicated by I. Potrykus

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Pérez, C., Fernández, B. & Rodríguez, R. In vitro plantlet regeneration through asexual embryogenesis in cotyledonary segments of Corylus avellana L.. Plant Cell Reports 2, 226–228 (1983). https://doi.org/10.1007/BF00269146

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  • DOI: https://doi.org/10.1007/BF00269146

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