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Improved culture ability of potato protoplasts by use of activated charcoal

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Abstract

Protoplasts were obtained from in vitro grown plants of Solanum tuberosum L. The protoplasts were cultured in X-plate petri dishes with the culture medium joined to a reservoirmedium. When activated charcoal was added to the reservoirmedium the culture ability of the protoplasts was significantly increased. The effect of activated charcoal was mainly due to a less pronounced browning of the developing protoplasts and this technique might be of help in protoplast cultures where browning is a problem.

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Abbreviations

BAP:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxy-acetic acid

References

  • Anagnostakis, S.L. (1974) Planta 115: 281–283.

    Google Scholar 

  • Banks, M.S., Evans, P.K. (1976) Plant Sci. Lett. 7: 409–416.

    Google Scholar 

  • Binding, H. (1975) Physiol. Plant. 35: 225–227.

    Google Scholar 

  • Binding, H., Nehls, R., Schieder, O., Sopory, S.K., Wenzel, G. (1978) Physiol. Plant. 43: 52–54.

    Google Scholar 

  • Boekelmann, G.S., Roest, S. (1983) Z. Pflanzenphysiol. 109: 259–265.

    Google Scholar 

  • Fridborg, G., Eriksson, T. (1975) Physiol. Plant. 34: 306–307.

    Google Scholar 

  • Fridborg, G., Pedersén, M., Landström, L-E., Eriksson, T. (1978) Physiol. Plant. 43: 104–106.

    Google Scholar 

  • Gunn, R.E., Shepard, J.F. (1981) Plant Sci. Lett. 22: 97–101.

    Google Scholar 

  • Johansson, L., Andersson, B., Eriksson, T. (1982) Physiol. Plant. 54: 24–30.

    Google Scholar 

  • Karp, A., Nelson, R.S., Thomas, E., Bright, S.W.J. (1982) Theor. Appl. Genet. 63: 265–272.

    Google Scholar 

  • Kao, K.N., Michayluk, M.R. (1981) In vitro 17 (7): 645–648.

    Google Scholar 

  • Kohlenbach, H.W., Werniche, W. (1977) Z. Pflanzenphysiol. 86: 463–472.

    Google Scholar 

  • Menczel, L., Nagy, F., Kizz, Zs.R., Maliga, P. (1981) Theor. Appl. Genet. 59: 191–195.

    Google Scholar 

  • Murashige, T., Skoog, F. (1962) Physiol. Plant. 15: 473–497.

    Google Scholar 

  • Nakamura, A., Itagaki, R. (1973) Jpn. J. Breed. 23: 71–78.

    Google Scholar 

  • Shepard, J.F., Totten, R.E. (1977) Plant. Physiol. 60: 313–316.

    Google Scholar 

  • Shepard, J.F. (1980) In: Rubenstein, I., Gengenbach, B. Phillips, R.L., Green, C.E. (eds) Genetic Improvement of Crops: Emergent Techniques, University of Minnesota Press Minneapolis. pp. 185–219.

    Google Scholar 

  • Shepard, J.F. (1982) Plant Sci. Lett. 26: 127–132.

    Google Scholar 

  • Thomas, E. (1981) Plant Sci. Lett. 23: 81–86.

    Google Scholar 

  • Wenzel, G., Schieder, O., Przewozny, T., Sopory, S.K., Melchers, G. (1979) Theor. Appl. Genet. 55: 49–55.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Physiological Botany, University of Uppsala, Box 540, S-751 21, Uppsala, Sweden

    I. Carlberg & T. Eriksson

  2. Department of Genetics and Plant Breeding, Swedish University of Agricultural Sciences, Box 7003, S-750 07, Uppsala, Sweden

    K. Glimelius

Authors
  1. I. Carlberg
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  2. K. Glimelius
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  3. T. Eriksson
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Communicated by I. Potrykus

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Carlberg, I., Glimelius, K. & Eriksson, T. Improved culture ability of potato protoplasts by use of activated charcoal. Plant Cell Reports 2, 223–225 (1983). https://doi.org/10.1007/BF00269145

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  • DOI: https://doi.org/10.1007/BF00269145

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