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Protocatechuic acid production from trans-ferulic acid by Pseudomonas sp. HF-1 mutants defective in protocatechuic acid catabolism

  • Applied Microbiology
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Summary

A trans-ferulic acid-utilizing Pseudomonas sp. HF-1 was isolated from soil samples. Mutant HF-1124, capable of growing on trans-ferulic acid but not on protocatechuic acid, was isolated from HF-1 after mutagenesis with nitrosoguanidine. The optimum temperature was 30°C and the optimum pH was 7.0–8.0 for protocatechuic acid production from trans-ferulic acid by mutant HF-1124. Protocatechuic acid production reached 4 g/l from a concentration of 8 g/l trans-ferulic acid. As a result of co-oxidation of methoxy aromatic compounds by strain HF-1124 grown on acetic acid, protocatechuic acid was formed from vanillin and vanillic acid, and vanillic acid and isovanillic acid were formed from veratric acid. By the co-oxidative demethylation of substituted monomethoxybenzene, m- and p-hydroxybenzoic acids were accumulated from m-and p-anisic acid, respectively, while no products were detected from anisole, o-anisic acid, nitroanisole, methylanisole, methoxyphenol and dimethoxybenzene.

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References

  • Bernhardt FH, Erdin N, Staudinger H, Ullich V (1973) Interaction of substrates with a purified 4-methoxybenzoate monooxygenase system (O-demethylating) from Pseudomonas putida. Eur J Biochem 35:126–134

    Google Scholar 

  • Broadbent DA, Cartwright NJ (1974) Bacterial attack on phenolic ethers. Microbios 9:119–130

    Google Scholar 

  • Conkerton EJ, Chapital DC (1983) High-performance liquid chromatography separation of the cis-trans isomers of cinnamic acid derivatives. J Chromatogr 281:326–329

    Google Scholar 

  • Dardas A, Gal D, Barrelle M, Stauret-Ignazi G, Sterjiades R, Perrmont J (1985) The demethylation of guaiacol by a new bacterial cytochrome P-450. Arch Biochem Biophys 236:585–592

    Google Scholar 

  • Kirk TK, Higuchi T, Chang H (eds) (1980) Lignin biodegradation, vol. 1, CRC Press, Boca Raton, Fla, USA

    Google Scholar 

  • Stainer RY, Ralleroni NJ, Doudoroff M (1966) The aerobic Pseudomonas: a taxonomic study. J Gen Microbiol 43:159–271

    Google Scholar 

  • Toms A, Wood JM (1970) The degradation of trans-ferulic acid by Pseudomonas acidovorans. Biochemistry 9:337–343

    Google Scholar 

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Authors and Affiliations

  1. Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan

    Toshio Omori, Kazuhisa Hatakeyama & Tohru Kodama

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  1. Toshio Omori
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  2. Kazuhisa Hatakeyama
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  3. Tohru Kodama
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Omori, T., Hatakeyama, K. & Kodama, T. Protocatechuic acid production from trans-ferulic acid by Pseudomonas sp. HF-1 mutants defective in protocatechuic acid catabolism. Appl Microbiol Biotechnol 29, 497–500 (1988). https://doi.org/10.1007/BF00269075

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  • DOI: https://doi.org/10.1007/BF00269075

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