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Genetic analysis of unequal transmission of the mitochondrial markers in Saccharomyces cerevisiae

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Summary

The presence of mitochondrial sex factor, ω, was demonstrated in haploid strains of yeast Saccharomyces cerevisiae which came from our laboratory. Transmission and recombination of the mitochondrial genes (CR/CS, ER/ES and OR/OS), conferring the resistance/sensitivity to chloramphenicol, erythromycin and oligomycin, respectively, were non-polar in homosexual crosses and highly polar in heterosexual crosses.

Different results were obtained in crosses involving an erythromycin resistant mutant G706E11 (CSEROS) which was found to contain cellular DNA of diploid level. This strain was ω− and showed no recombination polarity when crossed to ω− haploid strains having the genotype CRESOR, but there was a highly polar transmission, that is, the alleles from G706E11 (CS, ER and OS) were transmitted to the zygote progeny in preference to the CR, ES and OR alleles. When crossed to ω+ haploid strains, there was a highly polar recombination, but no transmission was seen for the E and O alleles. Polar transmission of markers from ω+ haploid parental strain, characteristic of heterosexual crosses, was noticed only for the C allele. The crosses of G706E11 toω+ haploids featured an increase in the recombination frequency.

The values of % suppressiveness of ϱ − petite mutants were relatively low when determined by crossing to G706E11 or to ϱ + diploid strain M2-8C rather than by crossing to ϱ + haploid strains, indicating that there is a positive correlation between the polar transmission of drug resistance markers and the suppressiveness degrees.

Genetic mechanism of the anomalous behaviors if mitochondrial genes in crosses involving G706E11 was discussed and interpreted as due to an unbalanced supply of mitochondrial genomes from parental strains.

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  1. Central Research Laboratory, Mitsubishi Chemical Industries Limited, Yokohama, Japan

    Norio Gunge

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Communicated by F. Kaudewitz

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Gunge, N. Genetic analysis of unequal transmission of the mitochondrial markers in Saccharomyces cerevisiae . Molec. Gen. Genet. 139, 189–202 (1975). https://doi.org/10.1007/BF00268971

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