Summary
E. gracilis chloroplast DNA Bam fragments E and D, coding for rRNA were cloned separately using the plasmid pBR 322 as vector and E. coli as host. The newly constructed recombinant plasmids EgcKS 8 and EgcKS 11 (containing the Bam HI fragments E and D respectively) were analysed and characterized by gel electrophoresis, electronmicroscopy and analytical ultracentrifugation.
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Abbreviations
- Ap:
-
Ampicillin
- Tc:
-
Tetracycline-hydrochloride
- Bam HI:
-
endonuclease isolated from Bacillus amyloliquefaciens
- Eco RI:
-
endonuclease isolated from E. coli RY13
- Bgl II:
-
endonuclease isolated from Bacillus globiggi
- EDTA:
-
Ethylene-diamine-tetra-acetic-acid
- ctDNA:
-
chloroplast DNA
References
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Communicated by H. Boyer
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Knopf, U.C., Stutz, E. Molecular cloning of the gene region coding for the chloroplast rRNA of Euglena gracilis . Molec. Gen. Genet. 163, 1–6 (1978). https://doi.org/10.1007/BF00268957
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DOI: https://doi.org/10.1007/BF00268957