Skip to main content
SpringerLink
Log in

The genetic control of molybdoflavoproteins in Aspergillus nidulans

II. Use of the NADH dehydrogenase activity associated with xanthine dehydrogenase to investigate substrate and product induction

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

An NADH dehydrogenase activity is induced together with xanthine dehydrogenase I in Aspergillus nidulans wild type strains. The two activities have the same mobility in polyacrylamide gels (Fig.1) and are immunologically indistinguishable (Fig.2). Several hxB mutants which lack xanthine dehydrogenase activity but conserve the associated NADH dehydrogenase activity were used to determine that uric acid, but not hypoxanthine, is an inducer of the enzyme (Figs. 3 and 4). This fact together with results reported previously (Scazzocchio and Darlington, 1968) indicate that the induction of xanthine dehydrogenase I and urate oxidase requires the product specified by the uaY gene, as well as the common effector, urie acid.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

We’re sorry, something doesn't seem to be working properly.

Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Similar content being viewed by others

References

  • Alderson, T., Scazzocchio, C.: A system for the study of interlocus specificity for both forward and reverse mutation in at least eight gene loci in Aspergillus nidulans. Mutation Res. 4, 567–577 (1967)

    Google Scholar 

  • Arst, H.N., Jr., MacDonald, D.W., Cove, D.J.: Molybdate metabolism in Aspergillus nidulans. I. Mutations affecting nitrate reductase and or xanthine dehydrogenase. Molec. gen. Genet. 108, 129–145 (1970)

    Google Scholar 

  • Cove, D.J.: The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochim. biophys. Acta (Amst.) 113, 51–56 (1966)

    Google Scholar 

  • Cove, D.J.: Control of gene action in Aspergillus nidulans. Proc. roy. Soc. B 176, 267–275 (1970)

    Google Scholar 

  • Cove, D.J., Arst, H.N., Jr., Scazzocchio, C.: Regulation of gene activity in Aspergillus nidulans. In: Advances in molecular genetics (I. Falconer and D.J. Cove, eds.), vol. 1. London: Paul Elek (Scientific Books) Ltd. 1973 (in the press)

    Google Scholar 

  • Darlington, A.J., Scazzocchio, C.: Evidence for an alternative pathway of xanthine oxidation in Aspergillus nidulans. Biochim. biophys. Acta (Amst.) 166, 569–571 (1968)

    Google Scholar 

  • Darlington, A.J., Scazzocchio, C., Pateman, J.A.: Biochemical and genetical studies of purine breakdown in Aspergillus. Nature (Lond.) 206, 599–600 (1965)

    Google Scholar 

  • Hartley, M.J.: Mutation in Aspergillus. Ph. D. Thesis, University of Cambridge (1969)

  • Hartley, M.J.: Contrasting complementation patterns in Aspergillus nidulans. Genet. Res. 16, 123–125 (1970)

    Google Scholar 

  • Hayashi, S.I., Lin, E.C.C.: Product induction of glycerol kynase in Escherichia coli. J. molec. Biol. 14, 515–521 (1965)

    Google Scholar 

  • Hirschberg, R., Ensign, J.C.: Oxidation of nicotinic acid by a Bacillus species: Regulation of nicotinic acid and 6-hydroxynicotinic acid hydroxylases. J. Bact. 112, 392–397 (1972)

    Google Scholar 

  • Holl, F.B., Scazzocchio, C.: Immunological differences between inducible and constitutive xanthine dehydrogenases in Aspergillus nidulans. FEBS letters 12, 51–53 (1970)

    Google Scholar 

  • Jobe, A., Bourgeois, S.: lac repressor-operator interaction. VI. The natural inducer of the lac operon. J. molec. Biol. 69, 397–408 (1972)

    Google Scholar 

  • Palleroni, N.J., Stanier, R.Y.: Regulatory mechanisms governing synthesis of the enzymes for tryptophan oxidation by Pseudomonas fluorescens. J. gen. Microbiol. 35, 319–334 (1964)

    Google Scholar 

  • Pateman, J.A., Cove, D.J., Rever, B.M., Roberts, D.B.: A common co-factor for nitrate reductase and xanthine dehydrogenase which also regulates the synthesis of nitrate reductase. Nature (Lond.) 201, 58–60 (1964)

    Google Scholar 

  • Rajagopalan, K.V., Handler, P.: Purification and properties of chicken liver xanthine dehydrogenase. J. biol. Chem. 242, 4097–4107 (1967)

    Google Scholar 

  • Scazzocchio, C.: Studies on the genetic control of purine oxidation in Aspergillus nidulans. Ph. D. Thesis, University of Cambridge (1966)

  • Scazzocchio, C.: The genetic control of molybdoflavoproteins in Aspergillus nidulands: Allopurinol-resistant mutants constitutive for xanthine dehydrogenase. Appendix The induction of xanthine dehydrogenase II. Europ. J. Biochem. 36, 439–445 (1973)

    Google Scholar 

  • Scazzocchio, C., Darlington, A.J.: The genetic control of xanthine dehydrogenase and urate oxidase synthesis in Aspergillus nidulans. Bull. Soc. Chim. Biol. 49, 1503–1508 (1967)

    Google Scholar 

  • Scazzocchio, C., Darlington, A.J.: The induction and repression of the enzymes of purine breakdown in Aspergillus nidulans. Biochim. biophys. Acta (Amst.) 166, 557–568 (1968)

    Google Scholar 

  • Scazzocchio, C., Holl, F.B., Foguelman, A.I.: The genetic control of molybdoflavoproteins in Aspergillus nidulans: Allopurinol resistant mutants constitutive for xanthine dehydrogenase. Europ. J. Biochem. 36, 428–445 (1973)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Genetics, University of Cambridge, Cambridge, England

    Claudio Scazzocchio

Authors
  1. Claudio Scazzocchio
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by W. Gajewski

Paper I of this series is Scazzocchio, Holl and Foguelman (1973).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Scazzocchio, C. The genetic control of molybdoflavoproteins in Aspergillus nidulans . Molec. Gen. Genet. 125, 147–155 (1973). https://doi.org/10.1007/BF00268868

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00268868

Keywords

Search

Navigation