Skip to main content
Log in

Temperature-dependent conversion of sexual agglutinability in Saccharomyces cerevisiae

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Temperature dependency of sexual agglutinability in Saccharomyces cerevisiae was found. In almost all strains tested that were derived from several different sources, the agglutinability was constitutive when grown at 25° C but inducible when grown at 36° C, suggesting that the temperature-dependent conversion of sexual agglutinability is general nature in Saccharomyces. Cycloheximide and 8-hydroxyquinoline inhibited completely both cell division and the conversion of the agglutinability from constitutive to inducible type. N-Hydroxyurea and 5-fluorouracil which allowed cell growth to some extent inhibited the conversion slightly. Hence, the conversion of the agglutinability from constitutive to inducible type may be achieved in cells newly born after temperature shift. The reverse conversion of the agglutinability was gradual in comparison with the conversion from constitutive to inducible type. This conversion of the agglutinability was regulated by a single gene closely linked to mating type locus, which is recognizable by using a temperature-independent constitutive strain.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Betz, R., MacKay, V., Duntze, W.: a-factor from Saccharomyces cerevisiae: Partial characterization of a mating hormone produced by cells of mating type a. J. Bact. 132, 462–472 (1977)

    Google Scholar 

  • Brock, T.D.: Mating reaction in Hansenula wingei. Relation of cell surface properties to agglutination. J. Bact. 78, 59–68 (1959)

    Google Scholar 

  • Bücking-Throm, E., Duntze, W., Hartwell, L.H., Manney, T.R.: Reversible arrest of haploid yeast cells at the initiation of DNA synthesis by a diffusible sex factor. Exp. Cell Res. 76, 99–110 (1973)

    Google Scholar 

  • Calleja, G.B.: Flocculation in Schizosaccharomyces pombe. J. gen. Microbiol. 64, 247–250 (1970)

    Google Scholar 

  • Calleja, G.B., Johnson, B.F.: Flocculation in a fission yeast: an initial step in the conjugation process. Canad. J. Microbiol. 17, 1175–1177 (1971)

    Google Scholar 

  • Crandall, M.: Mating-type interactions in micro-organisms. In: Receptors and recognition (Cuatrecasas, P. and Greaves, M.F., eds.), Vol. 3, pp. 45–100, London: Chapman and Hall 1977

    Google Scholar 

  • Crandall, M.A., Brock, T.D.: Molecular basis of mating in the yeast Hansenula wingei. Bact. Rev. 32, 139–163 (1968)

    Google Scholar 

  • Doi, S., Yoshimura, M.: Temperature-sensitive loss of sexual agglutinability in Saccharomyces cerevisiae. Arch. Microbiol. 114, 287–288 (1977)

    Google Scholar 

  • Duntze, W., MacKay, V., Manney, T.R.: Saccharomyces cerevisiae: A diffusible sex factor. Science 168, 1472–1473 (1970)

    Google Scholar 

  • Egel, R.: Physiological aspects of conjugation in fission yeast. Planta (Berl.) 98, 89–96 (1971)

    Google Scholar 

  • Hagiya, M., Yoshida, K., Yanagishima, N.: The release of sex-specific substances responsible for sexual agglutination from haploid cells of Saccharomyces cerevisiae. Exp. Cell Res. 104, 263–272 (1977)

    Google Scholar 

  • Hartwell, L.H.: Synchronization of haploid yeast cell cycle, A predude to conjugation. Exp. Cell Res. 76, 111–117 (1973)

    Google Scholar 

  • Hawthorne, D.C.: A deletion in yeast and its bearing on the structure of the mating type locus. Genetics 48, 1727–1729 (1963)

    Google Scholar 

  • Hicks, J.B., Herskowitz, I.: Interconversion of yeast mating types. I. Direct observations of the action of the homothallism (HO) gene. Genetics 83, 245–258 (1976)

    Google Scholar 

  • Hicks, J.B., Herskowitz, I.: Interconversion of yeast mating types. II. Restoration of mating ability to sterile mutants in homothallic and heterothallic strains. Genetics 85, 373–393 (1977)

    Google Scholar 

  • Itoh, S., Takahashi, S., Tsuboi, M., Shimoda, C., Hayashibe, M.: Effect of light on sexual flocculation in Schizosaccharomyces japonicus. Plant and Cell Physiol. 17, 1355–1358 (1976)

    Google Scholar 

  • Lindegren, C.C., Lindegren, G.: A new method for hybridizing yeast. Proc. nat. Acad. Sci. (Wash.) 29, 306–308 (1943)

    Google Scholar 

  • Pomper, S., Burkholder, P.R.: Studies on the biochemical genetics of yeast. Proc. nat. Acad. Sci. (Wash.) 35, 456–464 (1949)

    Google Scholar 

  • Sakai, K., Yanagishima, N.: Mating reaction in Saccharomyces cerevisiae. I. Cell agglutination related to mating. Arch. Mikrobiol. 75, 260–265 (1971)

    Google Scholar 

  • Sakai, K., Yanagishima, N.: Mating reaction in Saccharomyces cerevisiae. II. Hormonal regulation of agglutinability of a type cells. Arch. Mikrobiol. 84, 191–198 (1972)

    Google Scholar 

  • Scherer, G., Hagg, G., Duntze, W.: Mechanism of α factor biosynthesis in Saccharomyces cerevisiae. J. Bact. 119, 386–393 (1974)

    Google Scholar 

  • Shimoda, C., Yanagishima, N.: Mating reaction in Saccharomyces cerevisiae. VI. Effect of 2-deoxyglucose on conjugation. Plant and Cell Physiol. 15, 767–778 (1974)

    Google Scholar 

  • Shimoda, C., Yanagishima, N.: Mating reaction in Saccharomyces cerevisiae. VIII. Mating-type-specific substances responsible for sexual cell agglutination. Antonie v. Leeuwenhoek 41, 521–532 (1975)

    Google Scholar 

  • Shimoda, C., Yanagishima, N., Sakurai, A., Tamura, S.: Mating reaction in Saccharomyces cerevisiae. IX. Regulation of sexual cell agglutinability of a type cells by a sex factor produced by α type cells. Arch. Microbiol. 108, 27–33 (1976)

    Google Scholar 

  • Wilkinson, L.E., Pringle, J.R.: Transient G1 arrest of S. cerevisiae cells of mating type α by a factor produced by cells of mating type a. Exp. Cell Res. 89, 175–187 (1974)

    Google Scholar 

  • Yamagishima, N.: Physiology of the mating reaction in yeast. Curr. Adv. Plant Sci. 7, 55–66 (1973)

    Google Scholar 

  • Yanagishima, N., Yoshida, K., Hamada, K., Hagiya, M., Kawanabe, Y., Sakurai, A., Tamura, S.: Regulation of sexual agglutinability in Saccharomyces cerevisiae of a and α types by sex-specific factors produced by their respective opposite mating types. Plant and Cell Physiol. 17, 439–450 (1976)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by F. Kaudewitz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Doi, S., Yoshimura, M. Temperature-dependent conversion of sexual agglutinability in Saccharomyces cerevisiae . Molec. Gen. Genet. 162, 251–257 (1978). https://doi.org/10.1007/BF00268850

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00268850

Keywords

Navigation