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The DNA sequence change resulting from the I Q1mutation, which greatly increases promoter strength

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Summary

DNA sequencing shows that the mutational alteration resulting from I Q1is a deletion of 15 base pairs in the lacI promoter. The deletion creates a greatly improved — 35 homology region, explaining the 50–100-fold increase in lac repressor synthesis.

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References

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Authors and Affiliations

  1. Départment de Biologie Moléculaire, Université de Genève, Genève, Switzerland

    Michèle P. Calos & Jeffrey H. Miller

Authors
  1. Michèle P. Calos
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  2. Jeffrey H. Miller
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Communicated by W. Arber

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Calos, M.P., Miller, J.H. The DNA sequence change resulting from the I Q1mutation, which greatly increases promoter strength. Molec. Gen. Genet. 183, 559–560 (1981). https://doi.org/10.1007/BF00268783

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  • DOI: https://doi.org/10.1007/BF00268783

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