Summary
The temperature-sensitive sporulation phenotype (Spots) of Bacillus subtilis RNA polymerase, ribosomal and protein synthesis elongation factor G mutations can be corrected by supplementing the growth medium with carbohydrates such as ribose or glycerol, or with synthetic lipids such as Tween 40. The data suggest that these mutations affect a single common aspect of developmental cell function. It is proposed that these lesions prevent sporulation by disturbing the regulation of sporulating cell metabolic balance.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Freese E, Lopez JM, Freese EB (1979) Initiation of bacterial and yeast sporulation by partial deprivation of guanine nucleotides. In: Koch G, Richter D (eds) Regulation of macromolecular synthesis by low molecular weight mediators. Academic Press, New York, pp 127–144
Hirochika H, Kobayashi Y (1978) Suppression of temperature-sensitive sporulation of a Bacillus subtilis elongation factor G mutation by RNA polymerase mutations. J Bacteriol 136:983–993
Leighton TJ (1973) An RNA polymerase mutation causing temperature-sensitive sporulation in Bacillus subtilis. Proc Natl Acad Sci USA 70:1179–1183
Leighton TJ (1974) Sporulation-specific translational discrimination in Bacillus subtilis. J Mol Biol 86:855–863
Leighton TJ (1974) New types of RNA polymerase mutations causing temperature-sensitive sporulation in Bacillus subtilis. J Biol Chem 252:268–272
Ouweneel WJ (1976) Developmental genetics of homoeosis. In: Caspari EW (ed) Advances in genetics, vol 18. Academic Press, New York, pp 179–248
Sharrock RA, Leighton T (1981) Intergenic suppressors of temperature-sensitive sporulation in Bacillus subtilis are allele non-specific. Mol Gen Genet 183:532–537
Sharrock RA, Leighton T, Wittmann HG (1981) Macrolide and aminoglycoside antibiotic resistance mutations in the Bacillus subtilis ribosome resulting in temperature-sensitive sporulation. Mol Gen Genet 183:538–543
Suzuki DT, Kaufman T, Falk D (1976) Conditionally expressed mutations in Drosophila melanogaster. In: Ashburner M, Novitski E (eds) The genetics and biology of Drosophila, vol 1 a. Academic Press, New York, pp 208–264
Tipper DJ, Johnson CW, Ginther CL, Leighton T, Wittmann HG (1977) Erythromycin resistant mutations in Bacillus subtilis cause temperature-sensitive sporulation. Mol Gen Genet 150:147–159
Wayne RR, Price CW, Leighton T (1981) Physiological suppression of the temperature-sensitive sporulation defect in a Bacillus subtilis RNA polymerase mutant. Mol Gen Genet 183:544–549
Author information
Authors and Affiliations
Additional information
Communicated by K. Isono
Rights and permissions
About this article
Cite this article
Rush Wayne, R., Leighton, T. Physiological suppression of Bacillus subtilis conditional sporulation phenotypes: RNA polymerase and ribosomal mutations. Molec. Gen. Genet. 183, 550–552 (1981). https://doi.org/10.1007/BF00268780
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00268780