Summary
Mutations c17 and ri c, which permit constitutive expression of genes O and P and transcriptional activation of λ DNA replication, act as new promoters since they map outside the immunity region and direct rightward transcription in the presence of repressor. In the λc17 P -or λri cP- lysogens, this constitutive transcription is restricted to the O-P region, and is at least one order of magnitude lower than that for an induced λP -lysogen. The operator-constitutive mutants v2 and v1v3 give very low levels of constitutive transcription from the l and r strands, respectively.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bøvre, K., Lozeron, H. A., Szybalski, W.: Multistep DNA-RNA hybridization techniques. In: Methods in virology, vol. 5 (K. Maramorosch and H. Koprowski, eds.). p. 57. New York: Academic Press 1971.
— Szybalski, W.: Patterns of convergent and overlapping transcription within the b2 region of coliphage λ. Virology 38, 614 (1969).
Campbell, A.: Sensitive mutants of bacteriophage λ. Virology 11, 22 (1961).
Davidson, N., Szybalski, W.: Physical and chemical characteristics of lambda DNA. In: The bacteriophage lambda (A. D. Hershey, ed.) p. 45. Cold Spring Harbor, N. Y.: Cold Spring Harbor Laboratory 1971.
Dove, W. F., Hargrove, E., Ohashi, M., Haugli, F., Guha, A.: Replicator activation in lambda. Jap. J. Genetics 44, Suppl. 1, 11 (1969).
—, Inokuchi, H., Stevens, W.: Replication control in phage lambda. In: The bacteriophage lambda (A. D. Hershey, ed.), p. 747. Cold Spring Harbor, N. Y.: Cold Spring Harbor Laboratory 1971.
Echols, H.: Regulation of lytic development. In: The bacteriophage lambda (A. D. Hershey, ed.), p. 247. Cold Spring Harbor, N. Y.: Cold Spring Harbor Laboratory 1971.
Fiandt, M., Hradecna, Z., Lozeron, H. A., Szybalski, W.: Electron micrographic mapping of deletions, insertions, inversions, and homologies in the DNAs of coliphages lambda and phi 80. In: The bacteriophage lambda (A. D. Hershey, ed.), p. 329. Cold Spring Harbor, N. Y.: Cold Spring Harbor Laboratory 1971.
Gingery, R., Echols, H.: Mutants of bacteriophage λ unable to integrate into the host chromosome. Proc. nat. Acad. Sci. (Wash.) 58, 1507 (1967).
Guarneros, G., Echols, H.: New mutants of bacteriophage lambda with a specific defect in excision from the host chromosome. J. molec. Biol. 47, 565 (1970).
Hopkins, N., Ptashne, M.: Genetics of virulence. In: The bacteriophage lambda (A. D. Hershey, ed.), p. 571. Cold Spring Harbor, N. Y.: Cold Spring Harbor Laboratory 1971.
Hradecna, Z., Szybalski, W.: Fractionation of the complementary strands of coliphage λ DNA based on the asymmetric distribution of poly I,G-binding sites. Virology 32, 633 (1967).
Jacob, F., Campbell, A.: Sur le système de répression assurant l'immunité chez les bactéries lysogènes. C. R. Acad. Sci. (Paris) 248, 3219 (1959).
—, Wollman, E. L.: Étude génetique d'un bactériophage tempère d'Escherichia coli. I. Le système génetique du bactériophage λ. Ann. Inst. Pasteur 87, 653 (1954).
Kaiser, A. D.: Mutations in a temperate bacteriophage affecting its ability to lysogenize E. coli. Virology 3 42 (1957).
—: Lambda DNA replication. In: The bacteriophage lambda (A. D. Hershey, ed.), p. 195. Cold Spring Harbor, N. Y.: Cold Spring Harbor Laboratory 1971.
— Jacob, F.: Recombination between related temperate bacteriophages and the genetic control of immunity and prophage localization. Virology 4, 509 (1957).
Kumar, S., Szybalski, W.: Transcription of the λdv plasmid and inhibition of λ phages in λdv carrier cells of Escherichia coli. Virology 41, 665 (1970).
Matsushiro, A.: Specialized transduction of tryptophan markers in Escherichia coli K12 by bacteriophage ϕ80. Virology 19, 475 (1963).
Nijkamp, H. J. J., Bøvre, K., Szybalski, W.: Controls of rightward transcription in coliphage λ. J. molec. Biol. 54, 599 (1970).
Nygaard, A. P., Hall, B. D.: Formation and properties of RNA-DNA complexes. J. molec. Biol. 9, 125 (1964).
Packman, W., Sly, W. S.: Constitutive λ DNA replication by λc17 a regulatory mutant related to virulence. Virology 34, 778 (1968).
Pereira da Silva L. H., Jacob, F.: Étude génétique d'une mutation modifiant la sensibilité a l'immunité chez le bactériophage λ. Ann. Inst. Pasteur 115, 2, 145 (1968).
Ptashne, M.: Repressor and its action: In: The bacteriophage lambda (A. D. Hershey, ed.), p. 221. Cold Spring Harbor, N. Y.: Cold Spring Harbor Laboratory 1971.
—, Hopkins, N.: The operators controlled by the λ phage repressor. Proc. nat. Acad. Sci. (Wash.) 60, 1282 (1968).
Sakakibara, Y., Tomizawa, J.: Regulation of transcription of lambda bacteriophage operator mutants. Virology 44, 463 (1971).
Sato, K., Nishimune, Y., Sato, M., Numich, R., Matsushiro, A., Inokuchi, H., Ozeki, H.: Suppression sensitive mutants of coliphage ϕ80. Virology 34, 637 (1968).
Signer, E. R.: Recombination between coliphages λ and ϕ80. Virology 22, 650 (1964).
—, Manly, K., Brunstetter, M.: Deletion mapping of the cIII-N region of bacteriophage λ. Virology 39, 137 (1969).
Szybalski, W., Bøvre, K., Fiandt, M., Guha, A., Hradecna, Z., Kumar, S., Lozeron, H. A., Maher, SR. V. M., Nijkamp, H. J. J., Summers, W. C., Taylor, K.: Transcriptional controls in developing bacteriophages. J. cell. Physiol. 74, Suppl. 1, 33 (1969).
Taylor, K., Hradecna, Z., Szybalski W.: Asymmetric distribution of the transcribing regions on the complementary strands of coliphage λ DNA. Proc. nat. Acad. Sci. (Wash.) 57, 1618 (1967).
Author information
Authors and Affiliations
Additional information
Communicated by G. Bertani
Aided in part by grants from the National Cancer Institute (CA-07175), the National Science Foundation (GB-2096) and the Netherland Organization for the Advancement of Pure Research, Z.W.O.
Rights and permissions
About this article
Cite this article
Nijkamp, H.J.J., Szybalski, W., Ohashi, M. et al. Gene expression by constitutive mutants of coliphage lambda. Molec. Gen. Genet. 114, 80–88 (1972). https://doi.org/10.1007/BF00268749
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00268749