Skip to main content
SpringerLink
Log in

Phenotypes represented by a mutational change in a 50s ribosomal protein component, 50-8, in Escherichia coli

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

An erythromycin resistant (ery r) mutant of Escherichia coli Q13, QE107, was characterized by (1) the cross resistance of the cells to several macrolide antibiotics such as erythromycin, tylosin, spiramycin, oleandomycin and leucomycin, (2) the reduced affinity of its ribosomes to erythromycin and probably to the other macrolides mentioned above, (3) a low peptidyl transferase activity of its ribosomes and (4) an altered 50-8 protein of the 50s ribosomal subunits. These characters were always transferred together with the ery marker in the transduction experiments.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Lapidot, Y., Dé Groot, N., Fry-Shafrir, I.: Modified aminoacyl-tRNA. II. A general method for the preparation of acylaminoacyl-tRNA. Biochim. biophys. Acta (Amst.) 145, 292–299 (1967),

    Google Scholar 

  • Nirenberg, M. W.: Cell-free protein synthesis directed by messenger RNA. In: Methods in enzymology, vol. VI, p. 17–23, ed. by S. P. Colowick and N. O. Kaplan. New York: Academic Press 1963.

    Google Scholar 

  • Otaka, E., Teraoka, H., Tamaki, M., Tanaka, K., Osawa, S.: Ribosomes from erythromycin resistant mutants of Escherichia coli Q13. J. molec. Biol. 48, 499–510 (1970).

    Google Scholar 

  • Pestka, S.: Studies on the formation of transfer ribonucleic acid-ribosome complex. III. The formation of peptide bonds by ribosomes in the absence of sueernatant enzymes. J. biol. Chem. 243, 2810–2820 (1968).

    Google Scholar 

  • Takata, R., Osawa, S., Tanaka, K., Teraoka, H., Tamaki, M.: Genetic studies of the ribosomal proteins in Escherichia coli. V. Mapping of erythromycin resistance mutations which lead to alteration of a 50s ribosomal protein component. Molec. gen. Genet. 109, 123–130 (1970).

    Google Scholar 

  • Tanaka, K., Teraoka, H.: Effect of erythromycin on polylysine synthesis directed by polyadenylic acid in an Escherichia coli cell-free system. J. Biochem. (Tokyo) 64, 635–648 (1968).

    Google Scholar 

  • — Tamaki, M., Otaka, E., Osawa, S.: Erythromycin-resistant mutant of Escherichia coli with altered ribosomal protein component. Science 162, 576–578 (1968).

    Google Scholar 

  • —, Watanabe, S., Osawa, S., Otaka, E., Takata, R.: Mutants of Escherichia coli resistant to macrolide antibiotics. In: Drug action and drug resistance in bacteria: Macrolide antibiotics and lineomycin, p. 201–213, ed. by S. Mitsuhashi. Tokyo: University of Tokyo Press 1971.

    Google Scholar 

  • Teraoka, H.: A reversible change in the ability of Escherichia coli ribosomes to bind to erythromycin. J. molec. Biol. 48, 511–515 (1970).

    Google Scholar 

  • —, Tamaki, M., Tanaka, K.: Peptidyl transferase activity of Escherichia coli ribosomes having an altered protein component in the 50s subunit. Biochem. biophys. Res. Commun. 38, 328–332 (1970).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Shionogi Research Laboratory, Shionogi & Co., Ltd., Fukushima-ku, Osaka, Japan

    Kentaro Tanaka, Hiroshi Teraoka & Mikio Tamaki

  2. Research Institute for Nuclear Medicine and Biology, Hiroshima University, Hiroshima, Japan

    Renkichi Takata & Syozo Osawa

  3. Department of Biochemistry and Biophysics Research Institute for Nuclear Medicine and Biology, Hiroshima University, Kasumi-Cho, Hiroshima, Japan

    Renkichi Takata & Syozo Osawa

Authors
  1. Kentaro Tanaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hiroshi Teraoka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mikio Tamaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Renkichi Takata
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Syozo Osawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by H. G. Wittmann

Preliminary data of part of this work has been published (Tanaka, Teraoka, Tamaki, Watanabe, Osawa, Otaka and Takata, 1971).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tanaka, K., Teraoka, H., Tamaki, M. et al. Phenotypes represented by a mutational change in a 50s ribosomal protein component, 50-8, in Escherichia coli . Molec. Gen. Genet. 114, 9–13 (1972). https://doi.org/10.1007/BF00268741

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00268741

Keywords

Search

Navigation