Summary
Streptomycin sensitive (ss) revertants were obtained from the streptomycin dependent mutant sd 3 by transfer to streptomycin-free medium. Contrary to ss-wildtype cells these ss-revertants were able to form new sd-colonies on streptomycin-medium. These secondary sd-cells were identical with the original sd 3-mutant. The frequency of formation of the secondary sd-colonies was different, it varied depending on the revertant strain from 0 to the remarkable number of 10−2. With increasing length of cultivation of the ss-revertants the capability of producing sd-cells decreased continuously. Finallym after 8 weeks the zero value was reached. The rate of decrease was depending on the revertant strain.
These results show, that most of the newly formed revertants in addition to ss-information copies still possessed sd-copies which were segregating in the process of cell-division and producing secondary sd-colonies on streptomycin-medium.
The sd-information is present in numerous copies; proved through the variable frequency of formation, the variable rate of decrease of sd-formation and the undiminished amount of segregation after cloning.
Sager and Ramanis have shown that the genes of the single chloroplasts of the Chlamydomonas-cell are present only in two copies. The sd 3-information is located on the more numerous mitochondria if the statement of Sager and Ramanis proves to be true for the vegetative cells in principle.
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Literatur
Arnold, C. G.: The induction of extrakaryotic mutations. In: Vogel und Röhrborn, Chemical mutagenesis in mammals and man. Berlin 1970.
Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoloxydase in Beta vulgaris. Plant Physiol. 24, 1–15 (1949).
Ebersold, W.T.: The isolation and characterization of some mutant strains of Chlamydomonas reinhardi Dang. Ph. D. Dissertation Stanford University (1954).
Eversole, R.A.: Biochemical mutants of Chlamydomonas reinhardi. Amer. J. Bot. 43, 404–407 (1956).
Gillham, N.W.: Induction of chromosomal and nonchromosomal mutations in Chlamydomonas reinhardi with N-Methyl-N′-nitro-N-nitrosoguanidine. Genetics 52, 529–537 (1965).
—: Uniparental inheritance in Chlamydomonas reinhardi. Amer. Natural. 103, 355–388 (1969).
— Levine, R.P.: Studies on the origin of streptomycin resistent mutants in Chlamydomonas reinhardi. Genetics 47, 1463–1474 (1962).
Gowans, C.S.: Genetic investigations on Chlamydomonas eugametos. Ph. D. Dissertation Stanford University (1957).
Hoober, J.K., Blobel, G.: Characterization of the chloroplastic and cytoplasmic ribosomes of Chlamydomonas reinhardi. J. molec. Biol. 41, 121–138 (1969).
Kessler, E., Czygan, F.C.: Physiologische und biochemische Beiträge zur Taxonomie der Gattung Chlorella. II. Untersuchungen an Mutanten. Arch. Mikrobiol. 54, 37–45 (1966).
Levine, R.P., Ebersold, W.T.: The relation of calcium and magnesium to crossing-over in Chlamydomonas reinhardi. Z. Vererbungsl. 89, 631–635 (1958).
Linnane, A.W., Saunders, G.W., Gingold, E.B., Lukins, H.B.: The biogenesis of mitochondria V. Cytoplasmic inheritance of erythromycin resistance in Saccharomyces cervisiae. Proc. nat. Acad. Sci. (Wash.) 59, 903–910 (1968).
McKinney, G.: Absorption of light by chlorophyll solutions. J. biol. Chem. 140, 315–322 (1941).
Müntz, K.: vergleichende Untersuchungen über die Bestimmung der Zellenzahl in Algenkulturen mit Zählkammer und automatischem Teilchenzähler (Coulter-Counter). Z. allg. Mikrobiol. 7, 187–196 (1967).
Nass, M.M.: Mitochondrial DNA: Advances, problems, and goals. Science 165, 25–35 (1969).
Ranneberg, H., Arnold, C.G.: Nicht-reziproke Rekombination als Erklärung aberranter Spaltungsresultate bei Chlamydomonas reinhardi. Molec. Gen. Genetics 102, 27–38 (1968).
Sager, R.: Mendelian and nonmendelian inheritance of streptomycin resistance in Chlamydomonas reinhardi. Proc. nat. Acad. Sci. (Wash.) 40, 356–363 (1954).
—: Streptomycin as a mutagen for non-chromosomal genes. Proc. nat. Acad. Sci. (Wash.) 48, 2018–2026 (1962).
— Hamilton, M.G.: Cytoplasmic and chloroplast ribosomes of Chlamydomonas. Ultracentrifugal characterization. Science 157, 709–711 (1967).
— Ramanis, Z.: The pattern of segragation of cytoplasmic genes in Chlamydomonas. Proc. nat. Acad. Sci. (Wash.) 61, 324–331 (1968).
—: A genetic map of non-mendelian genes in Chlamydomonas. Proc. nat. Acad. Sci. (Wash.) 65, 593–600 (1970).
— Tsubo, Y.: Genetic analysis of streptomycin resistance and—dependence in Chlamydomonas. Z. Vererbungsl. 92, 430–438 (1961).
—: Mutagenic effects of streptomycin in Chlamydomonas. Arch. Mikrobiol. 42, 159–175 (1962).
Schimmer, O., Arnold, C.G.: Untersuchungen zur Lokalisation eines außerkaryotischen Gens bei Chlamydomonas reinhardi. Arch. Mikrobiol. 66, 199–202 (1969).
Thomas, D.Y., Wilkie, D.: Inhibition of mitochondrial synthesis by erythromycin. Cytoplasmic and nuclear factors controlling resistance. Genet. Res. 11, 33–41 (1968a).
—: Recombination of mitochondrial drug-resistance factors in Saccharomyces cerevisiae. Biochem. biophys. Res. Commun. 30, 368–372 (1968b).
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Schimmer, O., Arnold, C.G. Untersuchungen über Reversions-und Segregationsverhalten eines außerkaryotischen Gens von Chlamydomonas reinhardii zur Bestimmung des Erbträgers. Molec. Gen. Genet. 107, 281–290 (1970). https://doi.org/10.1007/BF00268701
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DOI: https://doi.org/10.1007/BF00268701