Summary
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1)
We have constructed independent physical maps of the mtDNAs from three different wild-type Saccharomyces strains by double-digestion analysis and hybridization analysis, using restriction endonucleases EcoRI, HindII, HindIII, PstI, BamHI, Aval, HhaI, SalI and XhoI. Twentynine restriction enzyme sites have been localized on the mtDNA of Saccharomyces carlsbergensis, 47 on the mtDNA of Saccharomyces cerevisiae strain JS1-3D and 38 on the mtDNA of Saccharomyces cerevisiae strain KL14-4A.
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2)
Although the three DNAs show considerable differences in their fragmentation patterns with most nucleases tested, the overall sequence organization of the three maps of 30–40 fragments is identical. Differences in the maps can be explained by extra restriction enzyme recognition sites, possibly located on inserted pieces of DNA and by insertions and deletions.
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3)
Four major insertions (900, 1,500, 2,600 and 3,000 bp long) are found in KL14-4A mtDNA relative to Saccharomyces carlsbergensis mtDNA. These insertions are clustered in one quadrant of the mtDNA and account for the difference in size of these two mtDNAs (75,750 and 68,000 bp, respectively).
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Abbreviations
- SSC:
-
150 mM NaCl, 15 mM sodium citrate (pH 7.0)
- cRNA:
-
complementary RNA
- (k)bp:
-
(kilo)basepairs
- DTT:
-
dithiothreitol
- Py:
-
pyrimidine
- Pu:
-
purine
- fragment nomenclature:
-
see Methods
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Communicated by F. Kaudewitz
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Sanders, J.P.M., Heyting, C., Verbeet, M.P. et al. The organization of gene in yeast mitochondrial DNA. Molec. Gen. Genet. 157, 239–261 (1977). https://doi.org/10.1007/BF00268660
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DOI: https://doi.org/10.1007/BF00268660