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Small repeated sequences in the chloroplast genome of Chlamydomonas reinhardi

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Summary

Chloroplast DNA of Chlamydomonas reinhardi contains many inverted repeated sequences. Analysis by hydroxyapatite binding, S1 nuclease digestion, and electron microscopy indicates that these sequences are 0.1–0.3 kilobase pairs in length, are widely distributed in the chloroplast genome, and make up 4–7% of the chloroplast DNA.

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Abbreviations

RNA:

ribonucleic acid

rRNA:

ribosomal RNA

RNA:

complementary RNA

DNA:

deoxyribonucleic acid

chl DNA:

chloroplast DNA

HAP:

hydroxypatite

SSC:

0.15 M NaCl, 0.015 M sodium citrate

0.1xSSC, 2xSSC, 4.67xSSC:

0.1, 2, and 4.67 times the concentration of SSC, respectively

TCA:

trichloroacetic acid

PB:

NaPO4 buffer, pH 6.8

Kb:

Kilobase

KbP:

Kilobase pair

References

  • Bedbrook, J.R., Bogorad, L.: Endonuclease recognition sites mapped on Zea mays chloroplast DNA. Proc. Nat. Acad. Sci. (Wash.) 12, 4309–4313 (1976)

    Google Scholar 

  • Becbrook, J.R., Kolodner, R., Bogorad, L.: Zea mays chloroplast ribosomal RNA genes are part of a 22,000 base pair inverted repeat. Cell 11, 739–749 (1977)

    Google Scholar 

  • Behn, W., Herrmann, R.G.: Circular molecules of the β-satellite DNA of Chlamydomonas reinhardi. Molec. gen. Genet. 157, 25–30 (1977)

    Google Scholar 

  • Bolivar, F., Betlach, M.C., Heyneker, H.L., Shine, J., Rodriguez, R.L., Boyer, H.W.: Origin of replication of pBR345 plasmid DNA. Proc. Nat. Acad. Sci. (Wash.) 74, 5265–5269 (1977)

    Google Scholar 

  • Bolivar, F., Rodriguez, R.L., Greene, P.J., Betlach, M.C., Heyneker, H.L., Boyer, H.W., Crossa, J.H., Falkow, S.: Construction and characterization of new cloning vehicles II. A multipurpose cloning system. Gene 2, 95–113 (1977)

    Google Scholar 

  • Broker, T.R., Soll, L., Chow, L.T.: Underwound loops in selfrenatured DNA can be diagnostic of inverted duplications and translocated sequences. J. Mol. Biol. 113, 579–589 (1977)

    Google Scholar 

  • Chiang, K.-S., Sueoka, N.: Replication of chloroplast DNA in Chlamydomonas reinhardi during vegetative cell cycle: Its mode and regulation. Proc. Nat. Acad. Sci. (Wash.) 57, 1506–1513 (1967)

    Google Scholar 

  • Chilton, M.-D., Montoya, A.L., Merlo, D.J., Drummond, M.H., Nutter, R., Gordon, M.P., Nester, E.W.: Restriction endonuclease mapping of a plasmid that confers oncogenicity upon Agrobacterium tumefaciens strain B6-806. Plasmid 1, 254–269 (1978)

    Google Scholar 

  • Chow, L.T.: Sequence arrangements of the Escherichia coli chromosome and of putative insertion sequences, as revealed by electron microscopic heteroduplex studies. J. Mol. Biol. 113, 611–621 (1977)

    Google Scholar 

  • Chow, L.T., Kahmann, R., Kamp, D.: Electron microscopic characterization of DNAs of non-defective deletion mutants of bacteriophage Mu. J. Mol. Biol. 113, 591–609 (1977)

    Google Scholar 

  • Davis, R.W., Simon, M., Davidson, N.: Electron microscope heteroduplex methods for mapping regions of base sequence homology in neucleic acids. Meth. Enzymol. 21, 413–428 (1971)

    Google Scholar 

  • Denhardt, D.T.: A membrane-filter technique for The detection of complementary DNA. Biochem. Biophys. Res. Comm. 23, 641–646 (1966)

    Google Scholar 

  • Denniston-Thompson, K., Moore, D.D., Kruger, K.E., Furth, M.E., Blattner, F.R.: Physical structure of the replication origin of bacteriophage lambda. Science 198, 1051–1056 (1977)

    Google Scholar 

  • Dickson, R.C., Abelson, J., Barnes, W.M., Reznikoff, W.S.: Genetic regulation: The lac control region. Science 187, 27–35 (1975)

    Google Scholar 

  • Fiddes, J.C., Barrell, B.G., Godson, G.N.: Nucleotide sequence of the separate origins of synthesis of bacteriophage G4 viral and complementary DNA strands. Proc. Nat. Acad. Sci. (Wash.) 75, 1081–1085 (1978)

    Google Scholar 

  • Gelvin, S., Heizmann, P., Howell, S.H.: Identification and cloning of the chloroplast gene coding for the large subunit of ribulose-1, 5-bisphosphate carboxylase from Chlamydomonas reinhardi. Proc. Nat. Acad. Sci. (Wash.) 74, 3193–3197 (1977)

    Google Scholar 

  • Hermann, R.G., Bohnert, H.-J., Kowallik, K.V., Schmitt, J.M.: Size conformation and purity of chloroplast DNA of some higher plants. Biochim. Biophys. Acta 378, 305–317 (1975)

    Google Scholar 

  • Howell, S.H., Walker, L.L.: Informational complexity of the nuclear and chloroplast genomes of Chlamydomonas reinhardi. Biochim. Biophys. Acta 418, 249–256 (1976)

    Google Scholar 

  • Kolodner, R., tewari, K.K.: The molecular size and conformation of the chloroplast DNA from higher plants. Biochim. Biophys. Acta 402, 372–390 (1975)

    Google Scholar 

  • Manning, J.E., Richards, O.C.: Isolation and molecular weight of circular chloroplast DNA from Euglena gracilis. Biochim. Biophys. Acta 259, 285–296 (1972)

    Google Scholar 

  • Messer, W., Meijer, M., Bergmans, H.E.N., Hansen, F.G., von Meyerburg, K., Beck, E., Schaller, H.: Origin of replication, ori C, of the Escherichia coli K-12 chromosome: Nucleotide sequence. Cold Spring Harbor Symp. Quant. Biol. 43 (1978). In press

  • Ohtsubo, H., Ohtsubo, E.: Nucleotide sequence of an insertion element, IS 1. Proc. Nat. Acad. Sci. (Wash.) 75, 615–619 (1978)

    Google Scholar 

  • Rochaix, J.-D.: Studies with chloroplast DNA-plasmid hybrids from Chlamydomonas reinhardi. Genetics and Biogenesis of chloroplasts and mitochondria. (Th. Bücher, W. Neupert, W. Sebald, S. Werner eds.) p. 375. Amsterdam: North Holland Publishing Co. 1976

    Google Scholar 

  • Rochaix, J.-D., Malnoe, P.: Anatomy of chloroplast ribosomal DNA of Chlamydomonas reinhardi. Cell 15, 661–670 (1978)

    Google Scholar 

  • Southern, E.M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517 (1975)

    Google Scholar 

  • Studier, F.W.: Sedimentation studies of the size and shape of DNA. J. Mol. Biol. 11, 373–390 (1965)

    Google Scholar 

  • Sueoka, N., Chiang, K.-S., Kates, J.R.: Deoxyribonucleic acid replication in meiosis of Chlamydomonas reinhardi. I. Isotopic transfer experiments with a strain producing eight zoospores. J. Mol. Biol. 25, 47–66 (1967)

    Google Scholar 

  • Surzycki, S.J., Surzycki, J.A., Lutz, R.: In vitro transcription and translation of chloroplast DNA of C. reinhardi. Genetics and biogenesis of chloroplasts and mitochondria. (Th. Bücher, W. Neupert, W. Sebald, S. Werner eds.) p. 621. Amsterdam: North Holland Publishing Co. 1976

    Google Scholar 

  • Walbot, V.: The dimorphic chloroplasts of the C4 plant Panicum maximum contain identical genomes. Cell 11, 729–737 (1977)

    Google Scholar 

  • Wells, R., Sager, R.: Denaturation and renaturation kinetics of chloroplast DNA from Chlamydomonas reinhardi. J. Mol. Biol. 58, 611–622 (1971)

    Google Scholar 

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Author notes

  1. Stanton B. Gelvin

    Present address: Department of Microbiology and Immunology, School of Medicine, University of Washington, 98195, Seattle, Washington, USA

Authors and Affiliations

  1. Department of Biology, University of California, San Diego, 92093, La Jolla, California, USA

    Stanton B. Gelvin & Stephen H. Howell

Authors
  1. Stanton B. Gelvin
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  2. Stephen H. Howell
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Communicated by J. Schell

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Gelvin, S.B., Howell, S.H. Small repeated sequences in the chloroplast genome of Chlamydomonas reinhardi . Molec. Gen. Genet. 173, 315–322 (1979). https://doi.org/10.1007/BF00268642

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  • DOI: https://doi.org/10.1007/BF00268642

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