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Repair of gamma-ray induced lesions in E. coli cells deficient in DNA polymerase I and having thermosensitive DNA polymerase III

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Summary

A 2 hr-incubation of gamma-irradiated E. coli polA1 tsdnaE at 45° prior to plating at 30° results in the decrease of the fraction of viable bacteria. This effect is not observed in experiments with gamma-ray sensitive polA1 dnaE + and gamma-ray resistant polA1 + tsdnaE bacteria. The study of uptake of H3-thymine or H3-uridine into DNA or RNA of polA1 tsdnaE and polA1 dnaE + cells shows that the decrease in survival at 45° in the first strain after gamma-irradiation correlates with the DNA synthesis block rather than with the unspecific inhibition of RNA synthesis. DNA breakdown in gamma-irradiated polA1 tsdnaE cells was found to be higher at 45° as compared with 30°. The results may be interpreted as indicative of the participation of DNA-polymerase III in the repair of gamma-ray induced lesions possibly during replication.

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References

  • Bridges, B.A., Mottershead, R.: RecA-dependent mutagenesis occuring before DNA replication in UV- and γ-irradiated Escherichia coli. Mutation Res. 13, 1–8 (1971)

    Google Scholar 

  • Cooper, P.K., Hanawalt, P.C.: Role of DNA polymerase I and REC system in excision repair in Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 69, 1156–1160 (1972)

    Google Scholar 

  • Fangman, W.L., Russel, M.: X-irradiation sensitivity in Escherichia coli defective in DNA replication. Molec. gen. Genet. 110, 332–347 (1971)

    Google Scholar 

  • Gefter, M.L., Hirota, Y., Kornberg, T., Wechsler, J.A., Barnoux, C.: Analysis of DNA polymerase II and III in mutants of Escherichia coli thermosensitive for DNA synthesis. Proc. nat. Acad. Sci. (Wash.) 68, 3150–3153 (1971)

    Google Scholar 

  • Goebel, W.: Replication of the DNA of the colicinigenic factor E1 (col E1) at the restrictive temperature in a DNA replication mutant thermosensitive for DNA polymerase III. Nature (Lond.) (New Biol.) 237, 67–70 (1972)

    Google Scholar 

  • Green, M.H.L., Gray, W.J.L., Murden, J.A., Bridges, B.A.: Influence of growth phase on UV-induced lethality and DNA breakdown in a Kornberg polymerase deficient resA strain of Escherichia coli. Molec. gen. Genet. 112, 110–116 (1971)

    Google Scholar 

  • Howard-Flanders, P., Boyce, R.: DNA repair and genetic recombination: studies on mutants of Escherichia coli deficient in these processes. Radiat. Res., Suppl. 6, 165–184 (1966)

    Google Scholar 

  • Lucia, P. de, Cairns, J.: Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature (Lond.) 224, 1164–1166 (1969)

    Google Scholar 

  • Masker, W.E., Hanawalt, P.: Ultraviolet-stimulated DNA synthesis in toluenized Escherichia coli deficient in DNA polymerase I. Proc. nat. Acad. Sci. (Wash.) 70, 129–133 (1973)

    Google Scholar 

  • Okazaki, R., Arisawa, M., Sugino, A.: Slow joining of newly replicated DNA chains in DNA polymerase I-deficient Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 68, 2954–2957 (1971)

    Google Scholar 

  • Paterson, M.C., Boyle, J.M., Setlow, R.B.: Ultraviolet- and X-ray responses of a deoxyribonucleic acid polymerase-deficient mutant of Escherichia coli. J. Bact. 107, 61–67 (1971)

    Google Scholar 

  • Richardson, C.C., Inman, R.B., Kornberg, A.: Enzymic synthesis of deoxyribonucleic acid. XVIII. The repair of partially single-stranded DNA templates by DNA polymerase. J. molec. Biol. 9, 46–69 (1964)

    Google Scholar 

  • Sinzinis, B.I., Smirnov, G.B., Saenko, A.S.: Effect of polA1 mutation on the ability of Escherichia coli K12 to repair single strand breaks of DNA induced by methylmethane sulfonate. Biochim. biophys. Acta (Amst.) 247, 635–639 (1971)

    Google Scholar 

  • Smirnov, G.B., Filkova, E.V., Skavronskaya, A.G., Saenko, A.C., Sinzinis, B.I.: Loss and restoration of viability of E. coli due to combinations of mutations, affecting DNA polymerase I and repair activities. Molec. gen. Genet. 121, 139–150 (1973)

    Google Scholar 

  • Town, C.D., Smith, K.C., Kaplan, H.S.: DNA polymerase required for rapid repair of X-ray-induced DNA strand breaks in vivo. Science 172, 851–854 (1971)

    Google Scholar 

  • Youngs, D.A., Smith, K.C.: X-ray sensitivity and repair capacity of a polA1 exrA strain of Escherichia coli. K12. J. Bact. 114, 121–127 (1973)

    Google Scholar 

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Authors and Affiliations

  1. Institute of Cytology of the Academy of Sciences of the USSR, Leningrad, USSR

    N. V. Tomilin

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  1. N. V. Tomilin
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Communicated by B. A. Bridges

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Tomilin, N.V. Repair of gamma-ray induced lesions in E. coli cells deficient in DNA polymerase I and having thermosensitive DNA polymerase III. Molec. Gen. Genet. 129, 97–103 (1974). https://doi.org/10.1007/BF00268624

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  • DOI: https://doi.org/10.1007/BF00268624

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