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Production of Nicotiana tabacum x Nicotiana acuminata hybrid by ovule culture

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Abstract

Using a conventional sexual crossing technique, Nicotiana tabacum x N. acuminata was not produced. After the fertilized ovules were cultured for 20 days in a liquid Nitsch H medium, germination was observed. The roots grew rapidly but leaves did not. However, plantlets were produced in an H medium containing Benzyladenine or Kinetin (0.01–0.1 mg/l). The plantlets grew and flowered in a greenhouse. The chromosome number of the hybrid was 36 and its morphological characteristics were intermediate between those of parental species.

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Abbreviations

BA:

Benzyl adenine

K:

Kinetin

References

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Author notes

  1. Sumio Iwai

    Present address: Utsunomiya Experiment Station, Japan Tobacco Inc., 1900 Idei, Oyama, 323, Tochigi, Japan

  2. Kazuo Nakata

    Present address: Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo, Japan

  3. Nobumaro Kawashima

    Present address: Iwata Experiment Station, Japan Tobacco Inc., Iwata, P.O. Box 3, 438, Shizuoka, Japan

Authors and Affiliations

  1. Central Research Institute, Japan Tobacco Inc., 6-2 Umegaoka, Mldori-ku, 227, Yokohama, Kanagawa, Japan

    Sumio Iwai, Chie Kishi, Kazuo Nakata & Nobumaro Kawashima

Authors
  1. Sumio Iwai
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  2. Chie Kishi
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  3. Kazuo Nakata
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  4. Nobumaro Kawashima
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Additional information

Communicated by O. L. Gamborg

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Iwai, S., Kishi, C., Nakata, K. et al. Production of Nicotiana tabacum x Nicotiana acuminata hybrid by ovule culture. Plant Cell Reports 5, 403–404 (1986). https://doi.org/10.1007/BF00268613

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  • DOI: https://doi.org/10.1007/BF00268613

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