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Hybrids between tobacco crown gall cells and normal somatic cells of Atropa belladonna

Isolation and characterization of cell lines

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Abstract

Protoplast fusion of Nicotiana tabacum (B6S3) crown gall cells and Atropa belladonna leaf mesophyll cells was carried out. Hybrids were selected for their capacity to grow on hormone-free media and to green in light. Shoots incapable of rhizogenesis were regenerated on the same media and grafted onto normal plants of different species. 57 hybrid cell lines differing in their genetic constitution were produced. Analysis of hybrid lines involved the determination of the lysopine dehydrogenase (LpDH) activity and the molecular forms of esterase and amylase, a restriction analysis of chloroplast DNA and a cytogenetic study.

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Abbreviations

LS-H:

Linsmaier and Skoog (1965) hormone-free medium

LpDH:

lysopine dehydrogenase

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Authors and Affiliations

  1. Institute of Botany, Academy of Sciences of the Ukrainian SSR, Repina 2, 252601, Kiev, USSR

    Yuri Yu. Gleba, Ivan F. Kanevsky, M. V. Skarzhynskaya, I. K. Komarnitsky & N. N. Cherep

Authors
  1. Yuri Yu. Gleba
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  2. Ivan F. Kanevsky
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  3. M. V. Skarzhynskaya
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  4. I. K. Komarnitsky
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  5. N. N. Cherep
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Communicated by I. Potrykus

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Gleba, Y.Y., Kanevsky, I.F., Skarzhynskaya, M.V. et al. Hybrids between tobacco crown gall cells and normal somatic cells of Atropa belladonna . Plant Cell Reports 5, 394–397 (1986). https://doi.org/10.1007/BF00268611

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  • DOI: https://doi.org/10.1007/BF00268611

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