Abstract
Uniola paniculata L. is a major sand-dune stabilizing grass which is being utilized to prevent shoreline erosion. In vitro cultured caryopses of U. paniculata produced callus on MS medium supplemented with 22.5 μM 2,4-D, 4.4 μM BA and 87.6 mM sucrose. Shoot induction occurred after these calli were inoculated onto the same medium without 2,4-D. Rooting of in vitro-derived shoots occurred when transferred to a one-half strength MS medium containing 43.8 mM sucrose and 14.7 μM IBA. Plantlets were planted after the roots reached a length greater than 20 mm.
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Abbreviations
- BA:
-
N-(phenyl-methyl)-lH-purine-6-amine
- IBA:
-
lH-indole-3-butanoic acid
- MS:
-
Murashige and Skoog (1962)
- 2,4-D:
-
(2,4-dichlorophenoxy) acetic acid
- TC agar:
-
tissue culture agar, (K.C. Biologicals, Lenexa, KS)
- Subdue:
-
methaxyl:N-(2,6-dimethylphenyl)-N-(methyoxyacetyl) alanine methyl ester (CIBA- GEIGY)
References
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Woodhouse WW Jr, Seneca ED, Cooper AW (1968) Shore and Beach XX:15–21.
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Communicated by I. C. Phillips
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Hovanesian, J.C., Torres, K.C. Micropropagation of Uniola paniculata L.. Plant Cell Reports 5, 385–386 (1986). https://doi.org/10.1007/BF00268608
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DOI: https://doi.org/10.1007/BF00268608