Abstract
The initial and induced in vivo Nitrate Reductase Activity, the nitrate accumulation by in vitro-produced axillary shoots and plantlets of Pinus pinaster were compared respectively with those of shoots collected from seedlings and whole plants.
The usefulness of the nitrate of the medium used for in vitro axillary shoot formation is demonstrated by the occurrence of initial NR activity in the explants. When fed in a non in vitro situation with a 50 mM KNO3 solution, they have the same induced capacity to reduce nitrate as do shoots from seedlings, even though the latter accumulate less nitrate. Plants regenerated in vitro exhibit an ability to reduce nitrate similar to that of seedlings. In both types of plants, the Nitrate Reductase potential is greater in roots than in shoots.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- NR:
-
Nitrate Reductase
- BA:
-
6-Benzyladenine
References
Aslam M (1981) Plant Physiol 68: 305–308
Bigg WL, Daniel TW (1978) Plant Soil 50: 371–385
Bornman C, Vogelmann T (1984) Physiol Plant 61: 505–512
Brown CL, Sommer HE (1982) In: Bonga JM, Durzan DJ (eds) Tissue culture in forestry, Nijhoff, Junk, The Hague, pp 109–149
Conner LN, Conner AJ (1984) Plant Sci Lett 36: 241–246
David A (1982) In: Bonga JM, Durzan DJ (eds) Tissue culture in forestry, Nijhoff, Junk, The Hague, pp 72–108
David H, Isemukali K, David A (1978) C R Ac Sci Paris 287D: 245–248
Debergh PC (1983) Physiol Plant 59: 270–276
Faye M, Rancillac M, David A (1981) New Phytol 87: 557–565
Lawrence JM, Herrick HE (1982) Plant Sci Lett 24: 17–26
Martin F, Chemardin M, Gadal P (1981) Physiol Plant 53: 105–110
McKeand SE, Allen HL (1984) Physiol Plant 61: 523–528
Rancillac M, Faye M, David A (1982) Physiol Plant 56: 97–101
Robin P, Conejero G, Tranchant JP, Passama L, Salsac L, (1983) Physiol Veg 21: 123–128
Smirnoff N, Todd P, Steward GR (1984) Ann Bot 54: 363–374
Smirnoff N, Steward GR (1985) Physiol Plant 64: 133–140
Treguer P, Lecorre P (1975) Manuel d'analyses des sels nutritifs dans l'eau de mer, Université de Bretagne occidentale, Brest, France, pp 11–22
von Arnold S, Eriksson T (1984) Plant Cell Tissue Organ Culture 3: 257–264
Wetzstein HY, Sommer HE (1982) Amer J Bot 69: 1579–1586.
Author information
Authors and Affiliations
Additional information
Communicated by A. M. Boudet
Rights and permissions
About this article
Cite this article
Faye, M., David, A. & Lamant, A. Nitrate reductase activity and nitrate accumulation in in vitro produced axillary shoots, plantlets and seedlings of Pinus pinaster . Plant Cell Reports 5, 368–371 (1986). https://doi.org/10.1007/BF00268604
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00268604