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Production of the triterpenoid quassin in callus and cell suspension cultures of Picrasma quassioides Bennett

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Abstract

Plant cell and suspension cultures have been established from stem cuttings of Picrasma quassioides Bennett. The effect of 244 different types/concentrations of plant growth regulators on growth and quassin accumulation in callus tissue was investigated. Best growth, in terms of wet/dry weight after four weeks growth, was obtained on B5 media supplemented with 2% glucose, 10% coconut milk, 0.5 mg.l−1 zeatin riboside and 1.5 mg.l−1 IBA. The highest yields of quassin (0.014–0.018%) were detected on this same media supplemented with 1.0 mg.l−1 IBA and varying concentrations of zeatin riboside. Suspension cultures were easily established on B5 media supplemented with 2% glucose, 1.0 mg.l−1 2,4-D and 0.5 mg.l−1 kinetin. The carbon source had a marked effect on quassin accumulation with 0.32% quassin being detected when cells were grown in 2% galactose. This is comparable to the highest reported quassin yield for the whole plant.

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Abbreviations

IAA:

indole-3-acetic acid

IBA:

indolebutyric acid

IpA:

N6-(Δ-isopentenyl) adenine

IpAR:

N-(Δ-isopentenyl) adenine riboside

NAA:

naphthalene acetic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

6BA:

6-benzyladenine

References

  • Anderson L A, Harris A, Phillipson J D (1983) J Nat Prod 46: 374–378

    Google Scholar 

  • Constabel F, Shyluk J P, Gamborg O L (1971) Planta 96: 306–316

    Google Scholar 

  • Dix L, Van Staden J (1982) Plant Cell Tissue Organ Culture 1: 239–245

    Google Scholar 

  • Furuya T, Kojima H, Syonok (1971) Phytochem 10: 1529–1532

    Google Scholar 

  • Gamborg O L, Miller R A, Ojima K (1968) Expt. Cell Res 50: 151–158

    Google Scholar 

  • Hikino H, Ohta T, Takemoto T (1975) Phytochem. 14: 2473–2481

    Google Scholar 

  • London E, Robertson A, Worthington H (1950) J Chem Soc 3431

  • Murae T, Tsuyuki T, Ikeda T, Nishihama T, Masudo S, Takahashi T (1971) Tetrahedron: 27: 1545–1555 and 5147–5155.

    Google Scholar 

  • Pierre A, Robert-Gero M, Tempete C, Polonsky J (1980) Biochem Biophys Res Comm 93: 675–680.

    Google Scholar 

  • Polonsky J (1973a) Recent Advan Phytochem 6: 31–64

    Google Scholar 

  • Polonsky J (1973b) Fortschr Chem Org Naturst 30: 101–150

    Google Scholar 

  • Robins R J, Morgan M R A, Rhodes M J C, Furze J M (1984) Analytical Biochem 136: 145–156

    Google Scholar 

  • Skoog F, Miller C O (1957) Symp Soc Exp Biol 11: 118–131

    Google Scholar 

Download references

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Authors and Affiliations

  1. Wolfson Institute of Biotechnology, The University of Sheffield, S10 2TN, Sheffield, UK

    Alan H. Scragg & Eunice J. Allan

Authors
  1. Alan H. Scragg
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  2. Eunice J. Allan
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Communicated by W. Barz

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Scragg, A.H., Allan, E.J. Production of the triterpenoid quassin in callus and cell suspension cultures of Picrasma quassioides Bennett. Plant Cell Reports 5, 356–359 (1986). https://doi.org/10.1007/BF00268601

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  • DOI: https://doi.org/10.1007/BF00268601

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