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Analysis of characters coded for by T-DNA using hybridization between tumorous and normal plant cells

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Abstract

In somatic hybrids between tumourous Nicotiana tabacum (B6S3) and normal mesophyll Atropa belladonna cells, the following traits, directly or indirectly connected with T-DNA gene expression and tumourous growth, were analysed: lysopine dehydrogenase activity (LpDH), shoot suppression, root suppression, ability to grow on media with D-lactose as a sole carbon source and resistance to 2-aminoethylcysteine, 5-bromodeoxyuridine and 5-methyltryptophan. Dominant (semidominant) expression was observed for all but one trait studied, e.g. shoot suppression which behaved as a recessive character.

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Abbreviations

LS-H:

Linsmaier and Skoog (1965) hormone-free medium

NAA:

Naphtaleneacetic acid

BAP:

6-Benzylaminopurine

2,4-D:

2,4-Dichlorophenoxyacetic acid

5-BUdR:

5-Bromodeoxyuridine

2-AEC:

2-Aminoethylcysteine

5-MT:

5-Methyltryptophan

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Authors and Affiliations

  1. Institute of Botany, Academy of Sciences of the Ukrainian SSR, Repina 2, 252601, Kiev, USSR

    Ivan F. Kanevsky & Yury Yu. Gleba

Authors
  1. Ivan F. Kanevsky
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  2. Yury Yu. Gleba
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Communicated by I. Potrykus

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Kanevsky, I.F., Gleba, Y.Y. Analysis of characters coded for by T-DNA using hybridization between tumorous and normal plant cells. Plant Cell Reports 5, 352–355 (1986). https://doi.org/10.1007/BF00268600

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  • DOI: https://doi.org/10.1007/BF00268600

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