Skip to main content
SpringerLink
Log in

Physical mapping of the Xba I, Hinc II, Bgl II, Xho I, Sst I, and Pvu II restriction endonuclease cleavage fragments of mitochondrial DNA of S. cerevisiae

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

A detailed molecular dissection of the yeast mitochondrial genome can be made with restriction endonucleases that generate site-specific cuts in DNA. The ordering of restriction fragments provides the basis of the physical mapping of mitochondrial transcripts and antibiotic resistance (ant®) loci, and is a means of analyzing the molecular organization of mtDNA of petite and mit- deletion mutants.

We have previously mapped the sites in the mtDNA of yeast strain MH41-7B recognized by the endonucleases Eco RI, Hpa I, Hind III, Bam HI, Sal I, Pst I, and Hha I, providing a total of 41 cleavage sites. We have now mapped the sites recognized by the endonucleases Xba I, Hinc II, Bgl II, Pvu II, Xho I, and Sst I, which make 6, 13, 5, 6, 2, and 2 cuts, respectively. Fragment maps for each of these endonuclease sites were derived by analysis of the products of double-enzyme digests and by hybridization of 3H-cRNA probes transcribed from low-kinetic-complexity petite mtDNAs to restriction fragments generated by various combinations of enzymes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bernardi, G., Prunell, A., Fonty, G., Kopecka, H., Strauss, F.: The mitochondrial genome of yeast: Organization, evolution and the petite mutation. In: The genetic function of mitochondrial DNA (Saccone, C., Kroon, A.M., eds.) pp. 185–186. Amsterdam: Elsevier/North Holland Biomedical 1976

    Google Scholar 

  • Bolotin-Fukuhara, M., Fukuhara, H.: Modified recombination and transmission of mitochondrial genetic markers in rho- mutants of Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. U.S.A. 73, 4608–4612 (1976)

    Google Scholar 

  • Burgess, R.R.: A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase. J. Biol. Chem. 244, 6160–6167 (1969).

    Google Scholar 

  • Casey, J., Cohen, M., Rabinowitz, M., Fukuhara, H., Getz, G.S.: Hybridization of mitochondrial transfer DNAs with mitochondrial and nuclear DNA of grande (wild type) yeast. J. Mol. Biol. 63, 431–440 (1972)

    Google Scholar 

  • Gabain, A., von, Hayward, G.S., Bujard, H.: Physical mapping of the Hind III, Eco RI, Sal and Sma restriction endonuclease cleavage fragments from bacteriophage T5 DNA. Mol. Gen. Genet. 143, 279–290 (1976)

    Google Scholar 

  • Grivell, L.A., Moorman, A.F.M.: A structural analysis of the OXI 3 region on yeast mtDNA. In: Mitochondria 1977 genetics and biogenesis of mitochondria (Bandlow, W., Schweyen, R.J., Wolf, K., Kaudewitz, F., eds.), pp. 371–384. Berlin: W. de Gruyter 1977

    Google Scholar 

  • Heyting, C., Sanders, J.P.M.: The physical mapping of some genetic markers in the 21 s ribosomal region of the mitochondrial DNA of yeast. In: The genetic functions of mitochondrial DNA (Saccone, C., Kroon, A.M., eds.), pp. 273–280. Amsterdam: Elsevier/North-Holland Biomedical 1976

    Google Scholar 

  • Jacq, C., Kujawa, C., Grandchamp, C., Netter, P.: Physical characterization of the difference between yeast mtDNA alleles 22-1 and 22-2. In. Mitochondria 1977 genetics and biogenesis of mitochondria (Bandlow, W., Schweyen, R.J., Wolf, K., Kaudewitz, F., eds.), pp. 255–270. Berlin: W. de Gruyter 1977

    Google Scholar 

  • Lewin, A., Morimoto, R., Merten, S., Martin, N., Berg, P., Christianson, T., Levens, D., Rabinowitz, M.: Physical mapping of mitochondrial genes and transcripts in Saccharomyces cerevisiae. In: Mitochondria 1977 genetics and biogenesis of mitochondria (Bandlow, W., Schweyen, R.J., Wolf, K., Kaudewitz, F., eds.), pp. 271–289 Berlin: W. de Gruyter 1977

    Google Scholar 

  • Lewin, A., Morimoto, R., Rabinowitz, M., Fukuhara, H.: Restriction enzyme analysis of mitochondrial DNAs of petite mutants of yeast: Classification of petites, and deletion mapping of mitochondrial genes. Mol. Gen. Genet. 163, 257–275 (1978)

    Google Scholar 

  • Morimoto, R., Lewin, A., Hsu, H.J., Rabinowitz, M., Fukuhara, H.: Restriction endonuclease analysis of mitochondrial DNA from grande and genetically characterized cytoplasmic petite clones of Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. U.S.A. 72, 3862–3872 (1975)

    Google Scholar 

  • Morimoto, R., Lewin, A., Merten, S., Rabinowitz, M.: Restriction endonuclease mapping and analysis of grande and mutant yeast mitochondrial DNA. In: Genetics and biogenesis of chloroplasts and mitochondria (Bücher, T., Neupert, W., Sebald, W., Werner, S., eds.), pp. 519–524. Amsterdam: Elsevier/North-Holland Biomedical 1976

    Google Scholar 

  • Morimoto, R., Lewin, A., Rabinowitz, M.: Restriction cleavage map of mitochondrial DNA from the yeast Saccharomyces cerevisiae. Nucleic Acids Res. 7, 2331–2351 (1977)

    Google Scholar 

  • Morimoto, R., Lewin, A., Rabinowitz, M.: Physical mapping and characterization of the mitochondrial DNA and RNA sequences from mit- mutants defective in cytochrome oxidase peptide 1 (OXI 3). Mol. Gen. Genet. 170, 1–9 (1979)

    Google Scholar 

  • Morimoto, R., Merten, S., Lewin, A., Martin, N., Rabinowitz, M.: Physical mapping of genes on yeast mitochondrial DNA. Localization of antibiotic resistance loci, and rRNA and tRNA genes. Mol. Gen. Genet. 163, 241–255, (1978)

    Google Scholar 

  • Morimoto, R., Rabinowitz, M.: Physical mapping of the yeast mitochondrial genome: Derivation of the fine structure and gene map of strain D273-10B and comparison with a strain (MH41-7B) differing in genome size. Mol. Gen. Genet. 170, 25–48 (1979)

    Google Scholar 

  • Morimoto, R., Rabinowitz, M.: Physical analysis of recombination of yeast mitochondrial DNA. (Manuscript in preparation)

  • Ommen, G.J.B. van, Groot, G.S.P.: Transcription map of yeast mitochondrial DNA. In: Mitochondria 1977 genetics and biogenesis of mitochondria (Bandlow, W., Schweyen, R.J., Wolf, K., Kaudewitz, F., eds), pp. 415–424. Berlin: W. de Gruyter 1977

    Google Scholar 

  • Ommen, G.J.B. van, Groot, G.S.P., Borst, P.: Fine structure physical mapping of 4s RNA genes on mitochondrial DNA of Saccharomyces cerevisiae. Mol. Gen. Genet. 154, 255–262 (1977)

    Google Scholar 

  • Prunell, A., Bernardi, G.: The mitochondrial genome of wild-type yeast cells. VI. Genome organization. J. Mol. Biol. 110, 53–74 (1977b)

    Google Scholar 

  • Prunell, A., Kopecka, H., Strauss, F., Bernardi, G.: The mitochondrial genome of wild-type yeast cells. V. Genome evolution. J. Mol. Biol. 110, 17–52 (1977a)

    Google Scholar 

  • Rabinowitz, M., Jakovcic, S., Martin, N., Hendler, F., Halbreich, A., Lewin, A., Morimoto, R.: Transcription and organization of yeast mitochondrial DNA. In: The genetic function of mitochondrial DNA (Saccone, C., Kroon, A. M., eds.). pp. 219–230. Amsterdam: Elsevier/North-Holland Biomedical 1976

    Google Scholar 

  • Roberts, R.J., Myers, P.A., Morrison, A., Murray, K.: A specific endonuclease from Haemophilus haemolyticus. J. Mol. Biol. 103, 199–208 (1976)

    Google Scholar 

  • Sanders, J.P.M., Borst, P., Weijers, P.J.: The organization of genes in yeast mitochondrial DNA. II. The physical map of Eco RI and Hind II+III fragments. Mol. Gen. Genet. 143, 43–64 (1975)

    Google Scholar 

  • Sanders, J.P.M., Heyting, C., DiFranco, A., Borst, P., Slonimski, P.P.: The organization of genes in yeast mitochondrial DNA. In: The genetic function of mitochondrial DNA (Saccone, C., Kroon, A.M., eds.), pp. 259–272. Amsterdam: Elsevier/North-Holland Biomedical 1976a

    Google Scholar 

  • Sanders, J.P.M., Heyting, C., Borst, P.: The variability of the mitochondrial genome of Saccharomyces strains. In: Genetics and biogenesis of chloroplasts and mitochondria (Bücher, T., Neupert, W., Sebald, W., Werner, S., eds.), pp. 511–517. Amsterdam: Elsevier/North-Holland Biomedical 1976b

    Google Scholar 

  • Sanders, J.P.M., Heyting, C., Verbeet, M.P., Meijlink, F.C.P.W., Borst, P.: The organization of genes in yeast mitochondrial DNA. III. Comparison of the physical maps of the mitochondrial DNAs from three wild-type Saccharomyces strains. Mol. Gen. Genet. 157, 239–261 (1977)

    Google Scholar 

  • Sharp, P.A., Sugden, B., Sambrook, J.: Detection of two restriction endonuclease activities in Haemophilus parainfluenza using analytical agarose-ethidium bromide electrophoresis. Biochemistry 12, 3055–3063 (1973)

    Google Scholar 

  • Smith, H.O., Wilcox, K.: A restriction enzyme from Haemophilus influenza. I. Purification and general properties. J. Mol. Biol. 51, 379–391 (1970)

    Google Scholar 

  • Southern, E.M.: Transfer of DNA fragments to Millipore filters after restriction endonuclease cleavage followed by agarose gel electrophoresis. J. Mol. Biol. 98, 503–517 (1975)

    Google Scholar 

  • Thomas, M., Davis, R.W.: Studies on the cleavage of bacteriophage lambda DNA with Eco RI restriction endonuclease. J. Mol. Biol. 91, 315–328 (1957)

    Google Scholar 

  • Wilson, G.A., Young, F.E.: Isolation of a sequence-specific endonuclease (Bam I) from Bacillus amyloliquefaciens H. J. Mol. Biol. 97, 123–125 (1975)

    Google Scholar 

Download references

Author information

Author notes

  1. Richard Morimoto

    Present address: The Biological Laboratories, Harvard University, 02138, Cambridge, MA, USA

Authors and Affiliations

  1. Department of Medicine, The University of Chicago, 60637, Chicago, Illinois, USA

    Richard Morimoto & Murray Rabinowitz

  2. Department of Biochemistry, The University of Chicago, 60637, Chicago, Illinois, USA

    Richard Morimoto & Murray Rabinowitz

  3. Department of Biology, The University of Chicago, 60637, Chicago, Illinois, USA

    Richard Morimoto & Murray Rabinowitz

  4. The Franklin McLean Memorial Research Institute, 60637, Chicago, Illinois, USA

    Richard Morimoto & Murray Rabinowitz

Authors
  1. Richard Morimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Murray Rabinowitz
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by F. Kaudewitz

Fragment nomenclature: Fragments generated by restriction enzymes and separated by gel electrophoresis are designated by an abbreviation of the enzyme used (i.e., Bgl for Bgl II, Pvu for Pvu II) and numbered according to their mobility on the gels

This study was supported in part by grants HL04442 and HL09172 from the National Institutes of Health and the Louis Block Fund of The University of Chicago. The Franklin McLean Memorial Research Institute is operated by The University of Chicago for the United States Department of Energy under Contract No. EY-76-C-02-0069. R.M. was supported by USPHS Training Grant T32-6M-07197

Rights and permissions

Reprints and permissions

About this article

Cite this article

Morimoto, R., Rabinowitz, M. Physical mapping of the Xba I, Hinc II, Bgl II, Xho I, Sst I, and Pvu II restriction endonuclease cleavage fragments of mitochondrial DNA of S. cerevisiae . Molec. Gen. Genet. 170, 11–23 (1979). https://doi.org/10.1007/BF00268577

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00268577

Keywords

Search

Navigation