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Studies on the mechanism of the induction of site-specific recombination in the ade-6 locus of Schizosaccharomyces pombe

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Summary

This work reports the effect of the ade6 mutant M26 of the yeast Schizosaccharomyces pombe on intragenic three factor crosses. Three factor crosses of the kinds (a) M26 m 1 +/+ + m 2, (b) M26 + m 2/+ m 1 +, (c) + M26m 2/m 1 + + and (d) + M26 +/m 1 +/m 2 were done. The numbers of prototrophic recombinants were determined by plating free spores on selective media.

The data is congruent with the notion that the DNA at the M26 site undergoes frequent single strand breakage followed by extensive exonucleolytic degradation towards both ends of the locus. However, this is in and of itself insufficient to explain for example the occurrence of any prototrophs in the crosses M26 + m 2/+ m 1 + or + M26 m 2/m 1 + +. The results support the idea that DNA heteroduplexes are also formed and that the frequency with which these molecules are constructed is modified in the presence of M26. Although regions of hybrid DNA are formed, their synthesis is believed to result from secondary events relating to the resolution of a postulated transitional DNA triplex into double stranded configurations.

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  1. Department of Biology, The University of Toledo, Toledo, Ohio

    S. L. Goldman

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  1. S. L. Goldman
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Communicated by W. Gajewski

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Goldman, S.L. Studies on the mechanism of the induction of site-specific recombination in the ade-6 locus of Schizosaccharomyces pombe. Molec. Gen. Genet. 132, 347–361 (1974). https://doi.org/10.1007/BF00268574

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  • DOI: https://doi.org/10.1007/BF00268574

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