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Economizing DNA-specific labelling by deoxythymidine-5′-monophosphate in Saccharomyces cerevisiae

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Summary

Although strain 211-1 aM is able to take up 5′-dTMP it cannot overcome growth-inhibition caused by an aminopterin (APT)-sulfanilamide (SAA)-mediated block of thymidylate biosynthesis in the presence of exogenous 5′-dTMP. 11 mutants (T-strains) of strain 211-1 aM were isolated being able to grow under these conditions.

Genetical analysis of the T-strains revealed in each case the mutation of a single recessive gene, TYP, to be responsible for this character, and that the mutated genes belong to three complementation groups.

One such typ-mutant, strain 211-1 aMT2, studied in detail was found to utilize exogenous 5′-dTMP as poorly as strain 211-1 aM. However, when thymidylate-biosythesis is inhibited by APT plus SAA the utilization of 5′-dTMP is increased by a factor 2.5 as compared to conditions where the antimetabolites are absent.

Next we isolated a typ-mutant- derivative (typ tlr- mutant) utilizing exogenous 5′-dTMP better by a factor 2.5 than strain 211-1 aMT2. This typ tlr- mutant was made auxotrophic for 5′-dTMP. The efficiency of utilization of exogenous 5′-dTMP found for these typ tlr tmp-mutants was the same as that of their typ tlr- parental strain in the presence of APT plus SAA.

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Authors and Affiliations

  1. Arbeitsgruppe Mikrobengenetik im Fachbereich Biologie, Johann Wolfgang Goethe-Universität, Frankfurt a. Main, Germany

    Wolfgang W. Fäth & Martin Brendel

  2. Zentralinstitut für Biochemie und Biophysik, Freie Universität, Berlin, Germany

    Wolfgang Laskowski & Elke Lehmann-Brauns

Authors
  1. Wolfgang W. Fäth
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  2. Martin Brendel
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  3. Wolfgang Laskowski
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  4. Elke Lehmann-Brauns
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Communicated by F. Kaudewitz

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Fäth, W.W., Brendel, M., Laskowski, W. et al. Economizing DNA-specific labelling by deoxythymidine-5′-monophosphate in Saccharomyces cerevisiae. Molec. Gen. Genet. 132, 335–345 (1974). https://doi.org/10.1007/BF00268573

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  • DOI: https://doi.org/10.1007/BF00268573

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