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Effect of ultraviolet irradiation of bacteriophage f1 DNA on its conversion to replicative form by extracts of Escherichia coli

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Summary

When DNA of phage ϕX174 or phage f1 is used as a template after exposure to ultraviolet radiation, the conversion of single-stranded DNA to replicative form by cell-free extracts of Escherichia coli is inhibited. The extend of synthesis is proportional to the distance of a pyrimidine dimer from a specific origin of replication as calculated from the random location of dimers at various UV doses. The results therefore indicate that the initiation of DNA synthesis on these phage DNAs occurs normally at a specific site, and that chain elongation is blocked when replication reaches a photo product in the template. Reinitiation of DNA synthesis distal to the lesion does not occur.

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References

  • Abelson, J.A., Thomas, C.A., Jr.: The anatomy of the T5 bacteriophage DNA molecule. J. molec. Biol. 18, 262–291 (1966)

    Google Scholar 

  • Bazaral, M., Helinski, D.R.: Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli. J. molec. Biol. 36, 185–194 (1968)

    Google Scholar 

  • Benbow, R.M., Zuccarelli, A.J., Sinsheimer, R.L.: A role for singlestrand breaks in bacteriophage ϕX174 genetic recombination. J. molec. Biol. 88, 629–651 (1974)

    Google Scholar 

  • Bollum, F.J., Setlow, R.B.: Ultraviolet inactivation of DNA primer activity. I. Effects of different wavelength and doses. Biochem. biophys. Acta (Amst.) 68, 599–607 (1963)

    Google Scholar 

  • Braun, A., Grossman, L.: An endonuclease from Escherichia coli that acts preferentially on UV-irradiated DNA and is absent from the uvrA and uvrB mutants. Proc. nat. Acad. Sci. (Wash.) 71, 1838–1842 (1974)

    Google Scholar 

  • Campbell, J.L., Soll, L., Richardson, C.C.: Isolation and partial characterization of a mutant of Escherichia coli deficient in DNA polymerase II. Proc. nat. Acad. Sci. (Wash.) 69, 2090–2094 (1972)

    Google Scholar 

  • Cohen, S.N., Chang, A.C.Y., Hsui, L.: Nonchromosomal antibiotic resistance in bacteria: Genetic transformation of Escherichia coli by R-factor DNA. Proc. nat. Acad. Sci. (Wash.) 69, 2110–2114 (1972)

    Google Scholar 

  • David, C.N.: UV inactivation and thymine dimerization in bacteriophage ϕX174. Z. Vererbungsl. 95, 318–325 (1964)

    Google Scholar 

  • Gefter, M.L., Hirota, Y., Kornberg, T., Wechsler, J.A., Barnoux, C.: Analysis of DNA polymerases II and III in mutants of Escherichia coli thermosensitive for DNA synthesis. Proc. nat. Acad. Sci. (Wash.) 68, 3150–3153 (1971)

    Google Scholar 

  • Grossman, L.: Enzymes involved in the repair of DNA. Adv. in Radiation Biol. (J.T. Lett, H. Adler, and M. Zelle, eds.), Vol. 4, pp. 77–129. Academic Press: New York and London 1974

    Google Scholar 

  • Hirota, Y., Gefter, M.L., Mindich, L.: A mutant of Escherichia coli defective in DNA polymerase II activity. Proc. nat. Acad. Sci. (Wash.) 69, 3238–3242 (1972)

    Google Scholar 

  • Howard-Flanders, P.: DNA repair. Ann. Rev. Biochem. 37, 175–200 (1968)

    Google Scholar 

  • Howard-Flanders, P., Boyce, R.P., Theriot, L.: Three loci in Escherichia coli K12 that control the excision of pyrimidine dimers and certain other mutagenic products from DNA. Genetics 53, 1119–1136 (1966)

    Google Scholar 

  • Kanner, L., Hanawalt, P.: Repair deficiency in a bacterial mutant defective in DNA polymerase. Biochem. biophys. Res. Commun. 39, 149–155 (1970)

    Google Scholar 

  • Klein, A., Bonhoeffer, F.: DNA replication. Ann. Rev. Biochem. 41, 301–332 (1972)

    Google Scholar 

  • Knippers, R.: DNA polymerase II. Nature (Lond.) 228, 1050–1053 (1970)

    Google Scholar 

  • Kornberg, T., Gefter, M.L.: DNA synthesis in cell-free extracts of a DNA polymerase defective mutant. Biochem. biophys. Res. Commun. 40, 1348–1355 (1970)

    Google Scholar 

  • Kornberg, T., Gefter, M.L.: Purification and DNA synthesis in cell-free extracts: Properties of DNA polymerase II. Proc. nat. Acad. Sci. (Wash.) 68, 761–764 (1971)

    Google Scholar 

  • Ling, V.: Pyrimidine sequences from the DNA of bacteriophage fd, f1, and ϕX174. Proc. nat. Acad. Sci. (Wash.) 69, 742–746 (1972)

    Google Scholar 

  • Marvin, D.A., Hohn, B.: Filamentous bacterial viruses. Bact. Rev. 33, 172–209 (1969)

    Google Scholar 

  • Marvin, D.A., Schaller, H.: The topology of DNA from the small filamentous bacteriophage fd. J. molec. Biol. 15, 1–7 (1966)

    Google Scholar 

  • Moses, R.E., Richardson, C.C.: A new DNA polymerase activity of Escherichia coli. I. Purification and properties of the activity present in E. coli polAl. Biochem. biophys. Res. Commun 41, 1557–1564 (1970)

    Google Scholar 

  • Ono, J., Shimazu, Y.: Ultraviolet reactivation of a bacteriophage containing a single-stranded deoxyribonucleic acid as a genetic element. Virology 29, 295–302 (1966)

    Google Scholar 

  • Poddar, R.K., Sinsheimer, R.L.: Nature of the complementary strands synthesized in vitro upon the single-stranded circular DNA of bacteriophage ϕX174 after ultraviolet irradiation. Biophys. J. 11, 355–369 (1971)

    Google Scholar 

  • Rauth, A.M.: The physical state of viral nucleic acid and the sensitivity of viruses to ultraviolet light. Biophys. J. 5, 257–273 (1965)

    Google Scholar 

  • Ray, D.S., Preuss, A., Hofschneider, P.H.: Replication of the singlestranded DNA of the male-specific bacteriophage M13. J. molec. Biol. 21, 485–491 (1966)

    Google Scholar 

  • Rupp, W.D., Howard-Flanders, P.: Discontinuities in the DNA synthesized in an excision-defective strain of Escherichia coli following ultraviolet irradiation. J. molec. Biol. 31, 291–304 (1968)

    Google Scholar 

  • Setlow, J.K.: Photoreactivation. Radiat. Res., Suppl. 6, 141–155 (1966)

    Google Scholar 

  • Setlow, R.B.: Macromolecular Radiosensitivity. Brookhaven Symposia in Biology, No. 14, p. 1–17 (1961)

  • Setlow, R.B., Setlow, J.K.: Evidence that ultraviolet-induced thymine dimers in DNA cause biological damage. Proc. nat. Acad. Sci. (Wash.) 48, 1250–1257 (1962)

    Google Scholar 

  • Sinsheimer, R.L.: A single-stranded deoxyribonucleic acid from bacteriophage ϕX174. J. molec. Biol. 1, 43–53 (1959)

    Google Scholar 

  • Sinsheimer, R.L.: Single-stranded DNA, ϕX174 DNA. In: Procedures in nucleic acid research (Cantoni and Davis, eds.), pp. 569–576. Harper and Row 1966

  • Stacey, K.A., Simson, E.: Improved method for the isolation of thymine-requiring mutants of Escherichia coli. J. Bact. 90, 554–555 (1965)

    Google Scholar 

  • Studier, F.W.: Sedimentation studies of the size and shape of DNA. J. molec. Biol. 11, 373–390 (1965)

    Google Scholar 

  • Sutherland, B.M., Court, D., Chamberlin, M.J.: Studies on the DNA photoreactivating enzyme from Escherichia coli. I. Transduction on the phr gene by bacteriophage lambda. Virology 48, 87–93 (1972)

    Google Scholar 

  • Tabak, H.F., Griffith, J., Geider, K., Schaller, H., Kornberg, A.: Initiation of deoxyribonucleic acid synthesis VII. A unique location of the gap in the M13 replicative duplex synthesized in vitro. J. biol. Chem. 249, 3049–3054 (1974)

    Google Scholar 

  • Tait, R.C., Harris, A.L., Smith, D.W.: DNA repair in Escherichia coli mutants deficient in DNA polymerases I, II, and/or III. Proc. nat. Acad. Sci. (Wash.) 71, 675–679 (1974)

    Google Scholar 

  • Van de Putte, P., van Sluis, C.A., van Dillewijn, J., Rorsch, A.: The location of genes controlling radiation sensitivity in Escherichia coli. Mutation Res. 2, 97–110 (1965)

    Google Scholar 

  • Vinograd, J., Lebowitz, J.: Physical and topological properties of circular DNA. J. gen. Physiol. 49, 103–126 (1966)

    Google Scholar 

  • Vinograd, J., Morris, J., Davidson, N., Dove, W.F.: The buoyant behavior of viral and bacterial DNA in alkaline CsCl. Proc. nat. Acad. Sci. (Wash.) 49, 12–17 (1963)

    Google Scholar 

  • Wickner, R.B., Ginsberg, B., Berkower, I., Hurwitz, J.: Deoxyribonucleic acid polymerase II of Escherichia coli. I. The purification and characterization of the enzyme. J. biol. Chem. 247, 489–497 (1972)

    Google Scholar 

  • Wickner, R.B., Ginsberg, B., Hurwitz, J.: Deoxyribonucleic acid polymerase II of Escherichia coli. II. Studies of the template requirements and the structure of the deoxyribonucleic acid product. J. biol. Chem. 247, 498–504 (1972a)

    Google Scholar 

  • Wickner, R.B., Wright, W., Wickner, S., Hurwitz, J.: Conversion of ϕX174 and fd single-stranded DNA to replicative forms in extracts of Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 69, 3233–3237 (1972b)

    Google Scholar 

  • Wickner, W.T., Brutlag, D., Schekman, R., Kornberg, A.: RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form. Proc. nat. Acad. Sci. (Wash.) 69, 965–969 (1972)

    Google Scholar 

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Author notes

  1. Yukito Masamune

    Present address: Faculty of Pharmaceutical Sciences, Kanazawa University, Takara-machi, (zip. 920), Kanazawa-city, Ishikawa-ken, Japan

Authors and Affiliations

  1. The Institute of Applied Microbiology, University of Tokyo, Tokyo, Japan

    Yukito Masamune

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  1. Yukito Masamune
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Communicated by T. Yura

Part of this work was supported by a Grant-in-Aid for scientific research from the Ministry of Education, Science and Culture, Japan.

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Masamune, Y. Effect of ultraviolet irradiation of bacteriophage f1 DNA on its conversion to replicative form by extracts of Escherichia coli . Molec. Gen. Genet. 149, 335–345 (1976). https://doi.org/10.1007/BF00268536

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  • DOI: https://doi.org/10.1007/BF00268536

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