Summary
The inheritance of an extrakaryotic mutation conferring temperature-sensitive growth on nonfermentable substrates and a high frequency of mutation to rho− has been studied. Multifactorial crosses (rho+xrho+) involving this mutation T S8 and mitochondrial mutations conferring resistance to chloramphenicol, erythromycin, oligomycin or paromomycin revealed: a) Mutation T S8 is localized on the mitDNA, referring to a new gene locus TSM1. b) Locus TSM1 appears to be weakly linked to the locus PAR1 and to the loci RIB1 and RIB3 but unlinked to the locus OLI1. c) The position of TSM1 is between PAR1 and the two closely linked loci RIB1 and RIB3, OLI1 is outside and not linked to the segment PAR-TSM-RIB. d) Mutation T S8 does not significantly influence the process of mitochondrial recombination and its control by the mitochondrial locus ω.
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Avner, P.R., Coen, D., Dujon, B., Slonimski, P.P.: Mitochondrial genetics IV: Allelism and mapping studies of oligomycin resistant mutants in S. cerevisiae. Molec. gen. Genet. 125, 9–52 (1973)
Bandlow, W., Schweyen, R.J.: On the mechanism of petite genesis in yeast IV: Biochemical characterization of a conditional cytoplasmic mutant producing petites at restrictive temperature. Biochem. biophys. Res. Commun. 67, 1078–1085 (1975)
Bolotin, M., Coen, D., Deutsch, J., Dujon, B., Netter, P., Slonimski, P.P.: La recombinaison des mitochondries chez Saccharomyces cerevisiae. Bull. Inst. Pasteur 69, 215–239 (1971)
Borst, P.: Mitochondrial nucleic acids. Ann. Rev. Biochem. 41, 333–376 (1972)
Casey, J., Gordon, P., Rabinowitz, M.: Characterization of mitochondrial deoxyribonucleic acid from grande and petite yeasts by renaturation and denaturation analysis and by transfer ribonucleic acid hybridization: evidence for internal repetition or heterogeneity in mitochondrial ribonucleic acid populations. Biochemistry 13, 1059–1066 (1974)
Coen, D., Deutsch, J., Netter, P., Petrochilo, E., Slonimski, P.P.: Mitochondrial genetics. I. Methodology and phenomenology. Soc. exp. Biol. Symp. 24, 449–496 (1970)
Cohen, M., Casey, J., Rabinowitz, M., Getz, G.S.: Hybridization of mitochondrial transfer RNA and mitochondrial DNA in petite mutants of yeast. J. molec. Biol. 63, 441–451 (1972)
Deutsch, J., Dujon, B., Netter, P., Petrochilo, E., Slonimski, P.P., Bolotin-Fukuhara, M., Coen, D.: Mitochondrial genetics VI: The petite mutation in Saccharomyces cerevisiae: Interrelations between the loss of the p + factor and the loss of the drug resistance mitochondrial genetic markers. Genetics 76, 195–219 (1974)
Dujon, B., Slonimski, P.P., Weill, L.: Mitochondrial genetics. VI. A model for recombination and segregation of mitochondrial genoms in Saccharomyces cerevisiae. Genetics 78, 415–437 (1974)
Dujon, B., Kruszewska, A., Slonimski, P.P., Bolotin-Fukuhara, M., Coen, D., Deutsch, J., Netter, P., Weill, L.: Mitochondrial genetics. X. Effects of UV irradiation on transmission and recombination of mitochondrial DNA in Saccharomyces cerevisiae. Molec. gen. Genet. 137, 29–72 (1975)
Dujon, B., Bolotin-Fukuhara, M., Coen, D., Deutsch, J., Netter, P., Slonimski, P.P., Weill, L.: Mitochondrial Genetics XI. Mutations at the mitochondrial locus ω affecting the recombination of mitochondrial genes in Saccharomyces cerevisiae. Molec. gen. Genet. 143, 131–165 (1976)
Faye, G., Fukuhara, H., Grandchamp, C., Lazowska, J., Michel, F., Casey, J., Getz, G.S., Locker, J. Rabinowitz, M., Bolotin-Fukuhara, M., Coen, D., Deutsch, J., Dujon, B., Netter, P., Slonimski, P.P.: Mitochondrial nucleic acids in the petite colonic mutants: deletion and repetition of genomes. Biochimie 55, 779–792 (1973)
Goldring, E.S., Grossman, L.J., Krupnick, D., Cryer, D.R., Marmur, J.: The petite mutation in yeast. Loss of mitochondrial deoxyribonucleic acid during induction of petites with ethidium bromide. J. molec. Biol. 52, 323–335 (1970)
Gordon, P., Casey, J., Rabinowitz, M.: Characterization of mitochondrial deoxyribonucleic acid from a series of petite yeast strains by deoxyribonucleic acid/deoxyribonucleic acid hybridization. Biochemistry 13, 1067–1075 (1974)
Gouhier-Monnerot, M.: Ethidium bromide resistance and enhancement of mitochondrial recombination. Molec. gen. Genet. 130, 65–79 (1974)
Heyting, C., Sanders, J. P. M.: The physical mapping of some genetic markers in the 21 s ribosomal region of the mitochondrial DNA of yeast. In: The genetic function of mitochondrial DNA (C. Saccone, A. M. Kroon, eds.) pp. 273–280. Amsterdam: North-Holland Press 1976
Hall, R.M., Nagley, P., Linnane, A. W.: Biogenesis of mitochondria XLII. Genetic analysis of the control of cellular mitochondrial DNA levels in Saccharomyces cerevisiae. Molec. gen. Genet. 145, 169–175 (1976)
Handwerker, A., Schweyen, R.J., Wolf, K., Kaudewitz, F.: Evidence for an extrakaryotic mutation affecting the maintenance of the rho factor in yeast. J. Bact. 113, 1307–1310 (1973)
Kleese, R., Grotbeck, R., Snyder, J.: Recombination among three mitochondrial genes in yeast (Sacccharomyces cerevisiae). J. Bact. 112, 1023–1025 (1972)
Kutzleb, R., Schweyen, R.J., Kaudewitz, F.: Extrachromosomal inheritance of paromomycin resistance in Saccharomyces cerevisiae: Genetic and biochemical characterization of mutants. Molec. gen. Genet. 125, 91–98 (1973)
Mahler, H.R.: Biogenetic autonomy of mitochondria. CRC Crit. Rev. Biochem. 1, 381–410 (1973)
Nagley, P., Gingold, E.B., Lukins, H.B., Linnane, A.W.: Biogenesis of mitochondria. XXV. Studies on the mitochondrial genomes of petite mutants in yeast using ethidium bromide as a probe. J. molec. Biol. 78, 335–350 (1973)
Nagley, P., Linnane, A.W.: Mitochondrial DNA deficient petite mutants of yeast. Biochem. biophys. Res. Commun. 39, 989–996 (1970)
Netter, P., Petrochilo, E., Slonimski, P.P., Bolotin-Fukuhara, M., Coen, D., Deutsch, J., Dujon, B.: Mitochondrial genetics. VII. Allelism and mapping studies of ribosomal mutants resistant to chloramphenicol, erythromycin and spiramycin in S. cerevisiae. Genetics 78, 1063–1100 (1974)
Sager, R.: Cytoplasmic genes and organelles. New York: Academic Press 1972
Saunders, G.W., Gingold, E.B., Trembath, M.H., Lukins, H.B., Linnane, A.W.: Mitochondrial genetics in yeast: Segregation of a cytoplasmic determinant in crosses and its loss or retention in the petite. In: Autonomy and biogenesis of mitochondria and chloroplasts (Bordman, H.K., Linnane, A.W., Smillie, R.M. eds.), pp. 185–193. Amsterdam: North Holland Press 1971
Schatz, G., Mason, T.L.: The biosynthesis of mitochondrial proteins. Ann. Rev. Biochem. 43, 51–88 (1974)
Schweyen, R.J., Steyrer, U., Kandewitz, F., Dujon, B., Slonimski, P.P.: Mapping of mitochondrial genes in S. cerevisiae: Population and pedigree analysis of retention or loss of four genetic markers on rho−. cells. 146, 131–165 (1976a)
Schweyen, R.J., Backhaus, B., Mathews, S., Kaudewitz, F.: Mapping by rho− deletion analysis and rho+xrho+ recombination analysis of mitochondrial genes in Saccharomyces cerevisiae. In: Genetics, biogenesis and bioenergetics of mitochondria., (W. Bandlow, R.J. Schweyen, D.Y. Thomas, K. Wolf, F. Kaudewitz, eds.) pp. 7–22. Berlin: De Gruyter 1976b
Schweyen, R.J., Weiss-Brummer, B., Backhaus, B., Kaudewitz, F.: Localization of seven gene loci on a circular map of the mitochondrial genome of Saccharomyces cerevisiae. In: The genetic function of mitochondrial DNA (C. Saccone and A.M. Kroon, eds.) pp. 251–258. Amsterdam: North-Holland Press 1976c
Sherman, R.: Respiration deficient mutants in yeast. I. Genetics. Genetics 48, 375 (1963)
Slonimski, P.P., Tzagaloff, A.: Localization in yeast mitochondrial DNA of mutations expressed in a deficiency of cytochrome oxidase and/or coenzyme QH2-cytochrome c reductase. Europ. J. Biochem. 61, 27–41 (1976)
Trembath, M.K., Monk, B.C., Kellerman, G.M., Linnane, A.W.: Biogenesis of mitochondria, 40: Phenotypic suppression of a mitochondrial mutation by a nuclear gene in Saccharomyces cerevisiae. Molec. gen. Genet. 140, 333–337 (1975)
Weislogel, P.O., Butow, R.A.: Low temperature and chloramphenicol induction of respiratory deficiency in a cold-sensitive mutant of Saccharomyces cerevisiae. Proc. nat. Acad. Sci. (Wash.) 67, 52–58 (1970)
Wolf, K., Dujon, B., Slonimski, P.P.: Mitochondrial genetics V: Multifactorial crosses involving a mutation conferring paromomycin-resistance in Saccharomyces cerevisiae. Molec. gen. Genet. 125, 53–90 (1973)
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Communicated by W. Gajewski
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Schweyen, R.J., Kaudewitz, F. On the formation of rho− petites in yeast. Molec. Gen. Genet. 149, 311–322 (1976). https://doi.org/10.1007/BF00268533
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DOI: https://doi.org/10.1007/BF00268533