Summary
An isogenic pair of Escherichia coli mutants (relA + tufB valS ts and relA1 tufB valS ts) has been cultured at several temperatures to establish various degrees of limitation for valyl-tRNA synthetase. The biosynthetic rate of 16 identifiable proteins, most of which are components of the transcription and translation apparatus, was measured by pulse-labelling with [35S]-methionine, followed by protein separation using two-dimensional gel electrophoresis (O'Farrell, 1975). No single pattern of response to amino acid starvation of biosynthetic rate was observed. EF-Ts, L12 and S6 were found to be under strong stringent and relaxed regulation; EF-G, EF-Tu-A and S1 are under strong stringent, but weak relaxed regulation; EF-Tu-B, α, VRS, IRS and ARS are under weak stringent and weak relaxed regulation; β is under weak stringent regulation and does not respond at all to relaxed conditions; the biosynthetic rate of a protein called stringent starvation protein is strongly stimulated, relative to other proteins, in the starved stringent strain.
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Abbreviations
- tRNA:
-
transfer RNA
- rRNA:
-
ribosomal RNA
- r proteins:
-
ribosomal proteins
- β:
-
β subunit of RNA polymerase
- α:
-
α subunit of RNA polymerase
- σ:
-
sigma factor of RNA polymerase
- EF-Tu-A:
-
elongation factor Tu encoded by tufA
- EF-Tu-B:
-
elongation factor Tu encoded by tufB
- EF-Ts:
-
elongation factor Ts
- EF-G:
-
elongation factor G
- EF-P:
-
elongation factor P
- VRS:
-
valyl-tRNA synthetase
- S1:
-
ribosomal protein S1
- S6:
-
ribosomal protein S6
- L12:
-
ribosomal protein L12
- SSP:
-
stringent starvation protein
- ppGpp:
-
guanosine-5′-diphosphate-3′-diphosphate
- pppGpp:
-
guanosine-5′-triphosphate-3′-diphosphate
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Communicated by H.G. Wittmann
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Reeh, S., Pedersen, S. & Friesen, J.D. Biosynthetic regulation of individual proteins in relA + and relA strains of Escherichia coli during amino acid starvation. Molec. Gen. Genet. 149, 279–289 (1976). https://doi.org/10.1007/BF00268529
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DOI: https://doi.org/10.1007/BF00268529