Summary
Ten different mutants with altered pigment patterns were isolated following X-irradiation of approximately 105 haploid protoplasts of Datura innoxia Mill. Seven of the selected strains gave rise to shoots and 3 to leaves only. The mutants were selected from light green or white calli, which had developed 4 weeks after transfer of developing cell clusters onto B5 agar medium containing 0.5 mg/l BAP (Gamborg et al., 1968). Of the 10 mutant strains 5 were light green, two were yellow, one was pale yellow and one was white. One additional strain does not possess anthocyanin in its stems; a feature chracteristic of the wildtype is the possession of anthocyanin. This strain is able to grow in soil and has now flowered. None of the mutants obtained is haploid. Nine are diploid and the other is tetraploid. The chlorophyll deficient strains can be propagated on B5 agar medium supplemented with higher concentration of sucrose than normally required for the growth of the wild-type.
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References
Binding H.: Cell cluster formation by leaf protoplasts from cultures of haploid Petunia hybrida L. Plant Sci. Letters 2, 185–188 (1974a)
Binding, H.: Regeneration von haploiden und diploiden Pflanzen aus Protoplasten von Petunia hybrida L. Z. Pflanzenphysiol. 74, 327–356 (1974b)
Binding, H.: Reproductibly high plating efficiencies of isolated mesophyll protoplasts from shoot cultures of tobacco. Physiol. Plant. 35, 225–227 (1975)
Durand, J., Potrykus, I., Donn, G.: Plantes issue de protoplastes de Pétunia. Z. Pflanzenphysiol. 69, 26–34 (1973)
Galun, E., Raveh, D.: In vitro culture of tobacco protoplasts: Survival of haploid and diploid protoplasts exposed to X-ray radiation at different times after isolation. Radiat. Botany 15, 79–82 (1975)
Gamborg, O.L., Miller, R.A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151–158 (1968)
Guha, S., Maheshwari, S.C.: In vitro production of embryos from anthers of Datura. Nature (Lond.) 204, 497 (1964)
Keller, W.A., Melchers, G.: The effect of high pH and calcium on tobacco leaf protoplast fusion. Z. Naturforsch. 28c, 737–741 (1973)
Melchers, G., Bergmann, L.: Untersuchungen an Kulturen von haploiden Geweben von Antirrhinum majus. Ber. dtsch. bot. Ges. 71, 459–473 (1959)
Melchers, G., Labib, G.: Somatic hybridisation of plants by fusion of protoplasts I. Selection of light resistent hybrids of “haploid” light sensitive varieties of tobacco. Molec. gen. Genet. 135, 277–294 (1974)
Schieder, O.: Regeneration von haploiden und diploiden Datura innoxia Mill. Mesophyll-Protoplasten zu Pflanzen. Z. Pflanzenphysiol. 76, 462–466 (1975)
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Communicated by G. Melchers
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Schieder, O. Isolation of mutants with altered pigments after irradiating haploid protoplasts from Datura innoxia Mill. with X-rays. Molec. Gen. Genet. 149, 251–254 (1976). https://doi.org/10.1007/BF00268525
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DOI: https://doi.org/10.1007/BF00268525