Plant Cell Reports

, Volume 6, Issue 3, pp 248–251 | Cite as

In vitro somatic embryogenesis and plant regeneration of cassava

  • László Szabados
  • Rodrigo Hoyos
  • William Roca


An efficient and reproducible plant regeneration system, initiated in somatic tissues, has been devised for cassava (Manihot esculenta Crantz). Somatic embryogenesis has been induced from shoot tips and immature leaves of in vitro shoot cultures of 15 cassava genotypes. Somatic embryos developed directly on the explants when cultured on a medium containing 4–16 mg/l 2,4-D. Differences were observed with respect to the embryogenic capacity of the explants of different varieties. Secondary embryogenesis has been induced by subculture on solid or liquid induction medium. Long term cultures were established and maintained for up to 18 months by repeated subculture of the proliferating somatic embryos. Plantlets developed from primary and secondary embryos in the presence of 0.1 mg/l BAP, 1mg/l GA3, and 0.01 mg/l 2,4-D. Regenerated plants were transferred to the field, and were grown to maturity.


Somatic Embryo Somatic Embryogenesis Plant Regeneration Induction Medium Shoot Culture 
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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • László Szabados
    • 1
  • Rodrigo Hoyos
    • 1
  • William Roca
    • 1
  1. 1.Biotechnology Research Unit, CIATCaliColombia

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