Molecular and General Genetics MGG

, Volume 179, Issue 1, pp 211–216 | Cite as

Genetic organization and restriction enzyme cleavage map of the ksgA-pdxA region of the Escherichia coli chromosome

  • Ólafur S. Andresson
  • Julian E. Davies


Small multicopy plasmids carrying the Escherichia coli genes ksgA and pdxA were constructed by ligation in vitro of an EcoRI restriction fragment from λksg10 (Andrésson and Davies, 1980a) into the EcoRI sites of the ColE1 plasmids RSF2124 and pVH51. Cleavage maps of the plasmids were determined for 21 different restriction enzymes. The ksgA gene was located in a 750 basepairs (bp) region 1,450 bp clockwise of the EcoRI site in folA; pdxA is in a 2,040 bp region immediately clockwise of ksgA.


Enzyme Escherichia Coli Restriction Enzyme Restriction Fragment Enzyme Cleavage 
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  1. Andrésson, Ó.S., Davies, J.E.: Lambda transducing phages for the E. coli genes kgsA and pdxA. Mol. Gen. Genet. 179, 201–209 (1980a)Google Scholar
  2. Andrésson, Ó.S., Davies, J.E.: Some properties of the ribosomal RNA methyltransferase ecoded by ksgA and the polarity of ksgA transcription. Mol. Gen. Genet. 179, 217–222 (1980b)Google Scholar
  3. Barnes, W.M.: Plasmid detection and sizing in single colony lysates. Science 195, 393–394 (1977)Google Scholar
  4. Berg, D.E., Davies, J., Allet, B., Rochaix, J.-D.: Transposition of R factor genes to bacteriophage. Proc. Natl. Acad. Sci. USA 72, 3628–3632 (1975)Google Scholar
  5. Bolivar, F., Rodriguez, R.L., Greene, P.J., Betlach, M.C., Heyneker, H.B., Boyer, H.W.: Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2, 95–113 (1977)Google Scholar
  6. Daniels, D.L., deWet, J.R., Blattner, F.R.: A genetic and physical map of bacteriophage λ DNA. J. Virol., in press (1980)Google Scholar
  7. Hardies, S.C., Patient, R.K., Klein, R.D., Ho, F., Reznikoff, W.S., Wells, R.D.: Construction and mapping of recombinant plasmids used for the preparation of DNA fragments containing the Escherichia coli lactose operator and promoter. J. Biol. Chem. 254, 5527–5534 (1979)Google Scholar
  8. Hershfield, V., Boyer, H.W., Chow, L., Helinski, D.R.: Characterization of a mini-ColE1 plasmid. J. Bacteriol. 126, 447–453 (1976)Google Scholar
  9. Humphreys, G.O., Willshaw, G.A., Anderson, E.S.: A simple method for the preparation of large quantities of pure plasmid DNA. Biochim. Biophys. Acta 383, 457–463 (1975)Google Scholar
  10. Iijima, T.: Studies on the colicinogenic factor in Escherichia coli K-12. Induction of colicin production by mitomycin C. Biken J. 5, 1–8 (1962)Google Scholar
  11. Jorgensen, R.A., Rothstein, S.J., Reznikoff, W.S.: A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance. Mol. Gen. Genet. 177, 65–72 (1979)Google Scholar
  12. Klein, R.D., Selsing, E., Wells, R.D.: A rapid microscale technique for isolation of recombinant plasmid DNA suitable for restriction enzyme analysis. Plasmid, in press (1980)Google Scholar
  13. Lennox, E.S.: Translation of linked genetic characters of the host by bacteriophage P1. Virology 1, 190–206 (1955)Google Scholar
  14. Mandel, M., Higa, A.: Calcium-dependent bacteriophage DNA infection. J. Mol. Biol. 43, 159–162 (1970)Google Scholar
  15. Meagher, R.B., Tait, R.C., Betlach, M., Boyer, H.W.: Protein expression in E. coli minicells by recombinant plasmids. Cell 10, 521–536 (1977)Google Scholar
  16. Miller, J.H.: Experiments in molecular genetics. New York: Cold Spring Harbor Laboratory 1972Google Scholar
  17. Poldermans, B., Roza, L., Van Knippenberg, P.H.: Studies on the function of two adjacent N6,N6-dimethyladenosines near the 3'end of 16S ribosomal RNA of Escherichia coli. III. Purification and properties of the methylating enzyme and methylase-30S interactions. J. Biol. Chem. 254, 9094–9100 (1979)Google Scholar
  18. Postle, K., Reznikoff, W.S.: HindII and HindIII restriction maps of the attϕ80-tonB-trp region of the Escherichia coli genome, and location of the tonB gene. J. Bacteriol. 136, 1165–1173 (1978)Google Scholar
  19. Roozen, D., Fenwick, R., Curtiss, R.: Synthesis of ribonucleic acid and protein in plasmid-containing minicells of Escherichia coli K-12. J. Bacteriol. 107, 21–33 (1971)Google Scholar
  20. So, M., Gill, R., Falkow, S.: The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA. Mol. Gen. Genet. 142, 239–249 (1975)Google Scholar
  21. Sutcliffe, J.G.: pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4,361 nucleotide pairs long. Nucleic Acids 5, 2721–2728 (1978)Google Scholar
  22. Tanaka, T., Weisblum, B.: Construction of a colicin El-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. J. Bacteriol. 121, 354–362 (1975)Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Ólafur S. Andresson
    • 1
  • Julian E. Davies
    • 1
  1. 1.Department of BiochemistryCollege of Agricultural and Life SciencesMadisonUSA

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