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Molecular and General Genetics MGG

, Volume 179, Issue 1, pp 211–216 | Cite as

Genetic organization and restriction enzyme cleavage map of the ksgA-pdxA region of the Escherichia coli chromosome

  • Ólafur S. Andresson
  • Julian E. Davies
Article

Summary

Small multicopy plasmids carrying the Escherichia coli genes ksgA and pdxA were constructed by ligation in vitro of an EcoRI restriction fragment from λksg10 (Andrésson and Davies, 1980a) into the EcoRI sites of the ColE1 plasmids RSF2124 and pVH51. Cleavage maps of the plasmids were determined for 21 different restriction enzymes. The ksgA gene was located in a 750 basepairs (bp) region 1,450 bp clockwise of the EcoRI site in folA; pdxA is in a 2,040 bp region immediately clockwise of ksgA.

Keywords

Enzyme Escherichia Coli Restriction Enzyme Restriction Fragment Enzyme Cleavage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Ólafur S. Andresson
    • 1
  • Julian E. Davies
    • 1
  1. 1.Department of BiochemistryCollege of Agricultural and Life SciencesMadisonUSA

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