Summary
Analysis of clear/turbid mottled (heterozygotic plaques) of Rhizobium meliloti temperate phage 16-3 indicates that the efficiency of repair at three sites (ti3, ti4, and ti5) in the C cistron is 2 to 20-fold less than that observed in E. coli phage λ. In agreement with this conclusion, heterozygotic plaques were observed at similar frequency in crosses where point and small delection mutants were combined, suggesting that in Rhizobium, DNA molecules with short single-stranded loops can escape from repair as efficiently as the simple mismatches.
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References
Amati, P., Meselson, M.: Localized negative interference in bacteriophage λ. Genetics 51, 369 (1965)
Campbell, A.: Genetic structure. In: The bacteriophage lambda (A.D. Hershey, ed.) p. 20. Cold Spring Harbor: Cold Spring Harbor Laboratory 1971
Dallmann, G., Orosz, L., Sain, B.: Restriction mapping of DNA of temperate Rhizobium meliloti phage 16-3: Comparison of genetic and physical maps indicates a long genetically silent chromosomal arm. Mol. Gen. Genet. 176, 439 (1979)
Holliday, R.: A mechanism for gene conversion in fungi. Genet. Res. 5, 282 (1964)
Kondorosi, Á., Kiss, G., Forrai, T., Vincze, É, Bánfalvi, ZS.: Circular linkage map of Rhizobium meliloti chromosome. Nature 268, 525 (1977)
Meselson, M., Radding, C.: A general model for genetic recombination. Proc. Natl. Acad. Sci. USA 72, 358 (1975)
Orosz, L., Rostás, K., Hotchkiss, R.D.: A comparison of two point, three point and deletion mapping in the C cistron of Rhizobium phage 16-3, and an explanation of the recombination pattern. Genetics 94 (1980) in press
Orosz, L., Sik, T.: Genetic mapping of Rhizobiophage 16-3. Acta Microbiol. Acad. Sci. Hung. 17, 185 (1970)
Orosz, L., Sváb, Z., Kondorosi, A., Sik, T.: Genetic studies on Rhizobiophage 16-3. I. Genes and functions on the chromosome. Mol. Gen. Genet. 125, 341 (1973)
Orosz, L.: Methods for analysis of the C cistron of temperate phage 16-3 of Rhizobium meliloti. Genetics 94 (1980) in press
Russo, V.E.A.: On the physical structure of lambda recombinant DNA. Mol. Gen. Genet. 122, 353 (1973)
White, R., Fox, M.: On the molecular basis of high negative interference. Proc. Natl. Acad. Sci. USA 71, 1544 (1974)
White, R., Fox, M.: Genetic consequences of transfection with heteroduplex bacteriophage λ DNA (Table 1 and 3). Mol. Gen. Genet. 141, 163 (1975)
Wildenberg, J., Meselson, M.: Mismatch repair in heteroduplex DNA. Proc. Natl. Acad. Sci. USA 72, 2202 (1975)
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Orosz, L., Páy, A. & Dallmann, G. Heterozygosis of phage 2-3 of Rhizobium meliloti: Moderate level of mismatch repair or gene conversion. Molec. Gen. Genet. 179, 163–167 (1980). https://doi.org/10.1007/BF00268459
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DOI: https://doi.org/10.1007/BF00268459