Skip to main content
SpringerLink
Log in

Mutation induction in yeast by deoxythymidine monophosphate: A model

  • Short Communication
  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

High concentrations of deoxythymidine monophosphate (dTMP) induce mutations in Saccharomyces cerevisiae. Strains defective in the RAD6 gene-thought to be involved in error-prone DNA repair-do not show dTMP-induced mutation. We propose a model to explain these findings and suggest that fluxes of thymidine nucleotides may diminish the fidelity of DNA replication.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Barclay BJ, Little JG (1978) Genetic damage during thymidylate starvation in Saccharomyces cerevisiae. Mol Gen Genet 160:33–40

    Google Scholar 

  • Bradley MO, Sharkey NA (1978) Mutagenicity of thymidine to cultured Chinese hamster cells. Nature 274:607–608

    Google Scholar 

  • Kunz BA, Barclay BJ, Game JC, Little JG, Haynes RH (1980) Induction of mitotic recombination in yeast by starvation for thymine nucleotides. Proc Natl Acad Sci USA 77:6057–6061

    Google Scholar 

  • Langjahr UG, Hartmann EA, Brendel M (1975) Nucleic acid metabolism in yeast I. Inhibition of RNA and DNA synthesis by high concentrations of exogenous deoxythymidine 5′-monophosphate in 5′-dTMP low requiring strains. Mol Gen Genet 143:113–118

    Google Scholar 

  • Lawrence CW, Stewart JW, Sherman F, Thomas FLX (1970) Mutagenesis in ultraviolet-sensitive mutants of yeast. Genetics 64:S36-S37

    Google Scholar 

  • Lawrence CW, Stewart JW, Sherman F, Christensen R (1974) Specificity and frequency of ultraviolet-induced reversion of an iso-1-cytochrome c ochre mutant in radiation-sensitive strains of yeast. J Mol Biol 85:137–162

    Google Scholar 

  • Little JG, Haynes RH (1979) Isolation and characterization of yeast mutants auxotrophic for 2′-deoxythymidine 5′-monophosphate. Mol Gen Genet 168:141–151

    Google Scholar 

  • Prakash L (1974) Lack of chemically induced mutation in repair-deficient mutants of yeast. Genetics 78:1101–1118

    Google Scholar 

  • Villani E, Boiteux S, Radman M (1978) Mechanism of ultraviolet-induced mutagenesis: Extent and fidelity of in vitro DNA synthesis on irradiated templates. Proc Natl Acad Sci USA 75:3037–3041

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, York University, 4700 Keele Street, M3J 1P3, Toronto, Ontario, Canada

    B. J. Barclay & J. G. Little

Authors
  1. B. J. Barclay
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. G. Little
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by G. O'Donovan

Rights and permissions

Reprints and permissions

About this article

Cite this article

Barclay, B.J., Little, J.G. Mutation induction in yeast by deoxythymidine monophosphate: A model. Molec. Gen. Genet. 181, 279–281 (1981). https://doi.org/10.1007/BF00268438

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00268438

Keywords

Search

Navigation