Summary
Mutant strains of Aspergillus nidulans have been isolated which grow normally on minimal medium at 37°C but not at 20°C. Growth tests indicate that these seventy-five mutant strains (designated CS, cold-sensitive) have a range of defects. Five are auxotrophic at 20°C, one (CS13) requiring isoleucine and another (CS48) choline. Many mutants are osmotic remedials. Some CS strains have altered properties at 37°C, including deoxycholate-sensitivity, actidione-resistance or actidione-ultrasensitivity.
The majority of thirty-two CS strains tested segregate cold-sensitivity as single gene mutations in crosses with wild-type. Cold-sensitivity of one strain (CS67) is cytoplasmically inherited. Dominance tests in heterokaryons showed that in each of twenty-six CS strains examined cold-sensitivity is determined by recessive mutations (designated cs). Complementation analysis of nine cs mutations showed that they each affect a different function. Crosses between some cs mutants, and the allocation of other cs mutations to different linkage groups, demonstrate that mutations to cold-sensitivity are not restricted to a limited region of the genome.
These results indicate that in Aspergillus nidulans selection for cold-sensitivity provides an enrichment for mutants with alterations in many different cellular properties.
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Waldron, C., Roberts, C.F. Cold-sensitive mutants in Aspergillus nidulans . Molec. Gen. Genet. 134, 99–113 (1974). https://doi.org/10.1007/BF00268413
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DOI: https://doi.org/10.1007/BF00268413