Skip to main content
Log in

Restriction fragment length polymorphism diversity in soybean

  • Originals
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Summary

Fifty-eight soybean accessions from the genus Glycine, subgenus Soja, were surveyed with 17 restriction fragment length polymorphism (RFLP) genetic markers to assess the level of molecular diversity and to evaluate the usefulness of previously identified RFLP markers. In general, only low levels of molecular diversity were observed: 2 of the 17 markers exhibited three alleles per locus, whereas all others had only two alleles. Thirty-five percent of the markers had rare alleles present in only 1 or 2 of the 58 accessions. Molecular diversity was least among cultivated soybeans and greatest between accessions of different soybean species such as Glycine max (L.) Merr. and G. soja Sieb. and Zucc. Principal component analysis was useful in reducing the multidimensional genotype data set and identifying genetic relationships.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Apuya N, Frazier B, Keim P, Roth EJ, Lark KG (1988) Restriction length polymorphisms as genetic markers in soybean, Glycine max (L.) Merr. Theor Appl Genet 75:889–901

    Google Scholar 

  • Carpenter JA, Fehr W (1986) Genetic variability for desirable agronomic traits in populations containing Glycine soja germplasm. Crop Sci 26:681–686

    Google Scholar 

  • Cianzio SR, Fehr WR (1987) Inheritance of agronomic and seed composition traits in Glycine max x Glycine soja crosses. J Agric Univ PR 1:53–63

    Google Scholar 

  • Delanney X, Rodgers DM, Palmer RG (1983) Relative genetic contribution among ancestral lines to North American soybean cultivars. Crop Sic 23:944–949

    Google Scholar 

  • Doyle JJ (1988) 5S Ribosomal gene variation in the soybean and its progenitor. Theor Appl Genet 75:621–624

    Google Scholar 

  • Doyle JJ, Beachy RN (1985) Ribosomal gene variation in soybean (Glycine) and its relatives. Theor Appl Genet 70:369–376

    Google Scholar 

  • Figdore SS, Kennard WC, Song KM, Slocum MK, Osborn TC (1988) Assessment of the degree of restriction fragment length polymorphism in Brassica. Theor Appl Genet 75:833–840

    Google Scholar 

  • Graef G (1988) Relation of isozyme genotypes to quantitative characters in soybean. PhD Diss, Iowa State University, Ames

    Google Scholar 

  • Griffin JD (1986) Genetic and germplasm studies with several isozyme loci in soybean [Glycine max (L.) Merr.]. PhD Diss, Iowa State University, Ames

    Google Scholar 

  • Groose RW, Weigelt HD, Palmer RG (1988) Somatic analysis of an unstable mutation for anthocyanin pigmentation in soybean. J Hered 79:263–267

    Google Scholar 

  • Hermann A (1962) A revision of genus Glycine and its immediate allies. USDA Tech Bull 1268:1–79

    Google Scholar 

  • Keim P, Shoemaker RC (1988) Construction of a random recombinant DNA library that is primarily single copy sequence. Soybean Genet Newslett 15:147–148

    Google Scholar 

  • Keim P, Olson TC, Shoemaker RC (1988) A rapid protocol for isolating soybean DNA. Soybean Genet Newslett 15:150–152

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NY

    Google Scholar 

  • Palmer RG, Kilen TC (1987) Qualitative genetics and cytogenetics. Soybeans: Improvement, production, and uses. Agronomy 16:135–209

    Google Scholar 

  • Quemada H (1986) The characterization of soybean 5S genes. PhD Diss University of Utah, Salt Lake City

    Google Scholar 

  • Roth JE, Frazier BL, Apuya NA, Lark KG (1989) Genetic variation in an inbred plant: Variation in tissue cultures of soybean. Genetics 121:359–368

    Google Scholar 

  • Specht JE, Williams JH (1984) Contribution of genetic technology to soybean productivity — retrospect and prospect. In: Fehr WR (ed) Genetic contributions to yield gains of five major crop plants. Am Soc Agron, Madison/WI, pp 49–74

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by A. L. Kahler

Rights and permissions

Reprints and permissions

About this article

Cite this article

Keim, P., Shoemaker, R.C. & Palmer, R.G. Restriction fragment length polymorphism diversity in soybean. Theoret. Appl. Genetics 77, 786–792 (1989). https://doi.org/10.1007/BF00268327

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00268327

Key words

Navigation