Summary
Fifty-eight soybean accessions from the genus Glycine, subgenus Soja, were surveyed with 17 restriction fragment length polymorphism (RFLP) genetic markers to assess the level of molecular diversity and to evaluate the usefulness of previously identified RFLP markers. In general, only low levels of molecular diversity were observed: 2 of the 17 markers exhibited three alleles per locus, whereas all others had only two alleles. Thirty-five percent of the markers had rare alleles present in only 1 or 2 of the 58 accessions. Molecular diversity was least among cultivated soybeans and greatest between accessions of different soybean species such as Glycine max (L.) Merr. and G. soja Sieb. and Zucc. Principal component analysis was useful in reducing the multidimensional genotype data set and identifying genetic relationships.
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References
Apuya N, Frazier B, Keim P, Roth EJ, Lark KG (1988) Restriction length polymorphisms as genetic markers in soybean, Glycine max (L.) Merr. Theor Appl Genet 75:889–901
Carpenter JA, Fehr W (1986) Genetic variability for desirable agronomic traits in populations containing Glycine soja germplasm. Crop Sci 26:681–686
Cianzio SR, Fehr WR (1987) Inheritance of agronomic and seed composition traits in Glycine max x Glycine soja crosses. J Agric Univ PR 1:53–63
Delanney X, Rodgers DM, Palmer RG (1983) Relative genetic contribution among ancestral lines to North American soybean cultivars. Crop Sic 23:944–949
Doyle JJ (1988) 5S Ribosomal gene variation in the soybean and its progenitor. Theor Appl Genet 75:621–624
Doyle JJ, Beachy RN (1985) Ribosomal gene variation in soybean (Glycine) and its relatives. Theor Appl Genet 70:369–376
Figdore SS, Kennard WC, Song KM, Slocum MK, Osborn TC (1988) Assessment of the degree of restriction fragment length polymorphism in Brassica. Theor Appl Genet 75:833–840
Graef G (1988) Relation of isozyme genotypes to quantitative characters in soybean. PhD Diss, Iowa State University, Ames
Griffin JD (1986) Genetic and germplasm studies with several isozyme loci in soybean [Glycine max (L.) Merr.]. PhD Diss, Iowa State University, Ames
Groose RW, Weigelt HD, Palmer RG (1988) Somatic analysis of an unstable mutation for anthocyanin pigmentation in soybean. J Hered 79:263–267
Hermann A (1962) A revision of genus Glycine and its immediate allies. USDA Tech Bull 1268:1–79
Keim P, Shoemaker RC (1988) Construction of a random recombinant DNA library that is primarily single copy sequence. Soybean Genet Newslett 15:147–148
Keim P, Olson TC, Shoemaker RC (1988) A rapid protocol for isolating soybean DNA. Soybean Genet Newslett 15:150–152
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NY
Palmer RG, Kilen TC (1987) Qualitative genetics and cytogenetics. Soybeans: Improvement, production, and uses. Agronomy 16:135–209
Quemada H (1986) The characterization of soybean 5S genes. PhD Diss University of Utah, Salt Lake City
Roth JE, Frazier BL, Apuya NA, Lark KG (1989) Genetic variation in an inbred plant: Variation in tissue cultures of soybean. Genetics 121:359–368
Specht JE, Williams JH (1984) Contribution of genetic technology to soybean productivity — retrospect and prospect. In: Fehr WR (ed) Genetic contributions to yield gains of five major crop plants. Am Soc Agron, Madison/WI, pp 49–74
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Communicated by A. L. Kahler
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Keim, P., Shoemaker, R.C. & Palmer, R.G. Restriction fragment length polymorphism diversity in soybean. Theoret. Appl. Genetics 77, 786–792 (1989). https://doi.org/10.1007/BF00268327
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DOI: https://doi.org/10.1007/BF00268327