Summary
A study has been made of the effects of a casamino acids shift-up on a prototrophic strain of yeast growing under conditions of ammonium repression. The shift-up produced an increase in growth rate some 120 min after the addition of amino acids to the medium. This growth rate increase was slightly preceded by an increase in the rate of accumulation of DNA. In contrast, the rate of accumulation of protein increased immediately and that of RNA 15–20 min after the shift. RNA was initially accumulated at a rate greater than that required to sustain the new steady state. This was shown to be due to an increase in the rate of synthesis of the rRNA species derived from the 35S precursor. The rate of synthesis of 5S rRNA and of tRNA increased much later and to a lesser extent than that of the 35S derived species. The implications of these results for general theories of the regulation of RNA synthesis are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barford, J.P., Hall, R.J.: Estimation of the length of cell cycle phases from asynchronous cultures of Saccharomyces cerevisiae. Exp. Cell Res. 102, 276–284 (1976)
Boehlke, K.W., Friesen, J.D.: Cellular content of ribonucleic acid and protein in Saccharomyces cerevisiae as a function of growth rate. J. Bact. 121, 429–439 (1975)
Bray, G.A.: A simple and efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analyt. Biochem. 1, 279–285 (1960)
Burton, K.: A study of the conditions and mechanism of the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 62, 315–323 (1956)
Dubois, E., Grenson, M., Wiame, J.: The participation of the anabolic glutamate dehydrogenase in nitrogen catabolite repression of arginase in Saccharomyces cerevisiae. Europ. J. Biochem. 48, 603–616 (1974)
Hartwell, L.H.: Macromolecule synthesis in temperature sensitive mutants of yeast. J. Bact. 93, 1662–1670 (1967)
Hartwell, L.H., Culotti, J., Pringle, J.R., Reid, B.J.: Genetic control of the yeast cell cycle. Science 183, 46–51 (1974)
Helser, T.L., McLaughlin, C.S.: Small ribonucleic acid molecules produced during ribosome biosynthesis in Saccharomyces cerevisiae. J. biol. Chem. 250, 2003–2007 (1975)
Herbert, P., Phipps, P.J., Strange, R.E.: Chemical analysis of microbial cells. Methods in Microbiol. 5, 209–344 (1971)
Leibowitz, R.D., Weinberg, R.A., Penman, S.: Unusual metabolism of 5S RNA in HeLa cells. J. molec. Biol. 73, 139–144 (1973)
Lowry, O.H., Roseborough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Ludwig, J.R. II: The effects of an amino acid shift-up on growth, macromolecular synthesis and phosphate metabolism in Saccharomyces cerevisiae. Master's Thesis, University of California, Irvine (1977)
Ludwig, J.R. II, Oliver, S.G., McLaughlin, C.S.: The effect of amino acids on growth and phosphate metabolism in a prototrophic yeast strain. Biochem. biophys. Res. Commun. (In press)
Maaløe, O.: The analysis of bacterial growth. Soc. Develop. Biol. Symp. (Suppl.) 3, 33–58 (1969)
Maxam, A.M., Tizard, R., Skryabin, K.G., Gilbert, W.: Promoter region for yeast 5S rRNA. Nature (Lond.) 267, 643–645 (1977)
McLaughlin, C.S., Magee, P.T., Hartwell, L.H.: Role of isoleucyl transfer ribonucleic acid synthetase in ribonucleic acid synthesis and enzyme repression. J. Bact. 100, 579–584 (1969)
Neidhardt, F.C.: The regulation of RNA synthesis in bacteria. Progr. Nucl. Acad Res. and Molec. Biol. 3, 145–181 (1964)
Nierlich, D.P.: Regulation of bacterial growth. Science 184, 1043–1050 (1974)
Oliver, S.G., McCready, S.J., Holm, C., Sutherland, P.A., McLaughlin, C.S., Cox, B.S.: Biochemical and physiological studies of the yeast virus-like particle. J. Bact. 130, 1303–1309 (1977)
Oliver, S.G., McLaughlin, C.S.: The regulation of RNA synthesis in yeast, I: Starvation experiments. Molec. gen. Genet. 154, 145–153 (1977)
Schneider, W.C.: Phosphorus compounds in animal tissues, I: Extraction and estimation of deoxypentose nucleic acid and pentose nucleic acids. J. biol. Chem. 161, 293–303 (1945)
Udem, S.A., Warner, J.R.: Ribosomal RNA synthesis in Saccharomyces cerevisiae. J. molec. Biol. 65, 227–242 (1972)
Waldron, C.: Synthesis of ribosomal and transter ribonucleic acids in yeast during a nutritional shift-up. J. gen. Microbiol. 98, 215–221 (1977)
Waldron, C., Lacroute, F.: Effect of growth rate on the amounts of ribosomal and transfer ribonucleic acids in yeast. J. Bact. 122, 855–865 (1975)
Watson, T.G.: Amino acid pool composition of Saccharomyces cerevisiae as a function of growth rate and amino acid nitrogen source. J. gen. Microbiol. 96, 263–268 (1976)
Wehr, C.T., Parks, L.W.: Macromolecular synthesis in Saccharomyces cerevisiae in different growth media. J. Bact. 98, 458–466 (1969)
Weinmann, R., Roeder, R.G.: Role of DNA dependent RNA polymerase III in the transcription of the tRNA and 5S RNA genes. Proc. nat. Acad. Sci. (Wash.) 71, 1790–1794 (1974)
Wiame, J.: The regulation of the biosynthesis and degradation of arginine in Saccharomyces cerevisiae. In: Proc. 3rd Int. Special Symp. in Yeast Metabolism and Cell Proc. (Soumalainen and Waller, eds.), Pt. II, pp. 307–330. Helsinki: Otariemi 1973
Zamb, T.J., Roth, R.: A modified diphenylamine assay quantitating DNA content. Yeast (Newsletter) 25, 140–142 (1977)
Author information
Authors and Affiliations
Additional information
Communicated by W. Gajewski
Paper I in this series is Oliver and McLaughlin (1977)
Rights and permissions
About this article
Cite this article
Richard Ludwig, J., Oliver, S.G. & McLaughlin, C.S. The regulation of RNA synthesis in yeast II: Amino acids shift-up experiments. Molec. Gen. Genet. 158, 117–122 (1977). https://doi.org/10.1007/BF00268303
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00268303