Summary
Genetic transformation experiments have been performed in Anacystis nidulans using donor material from two sources, namely chemically extracted DNA and extracellular nucleic acids. A high proportion of the transformants became mutant at sites which were wild type in both parental strains. Linkage was less extensive in transformation mediated by chemically extracted DNA, and this increased frequency of recombination was associated with enhanced mutation frequencies. The frequencies of recombination and mutation were varied to the same extent by changing the DNA concentration, and both processes were prevented by pretreatment of donor DNA with DNase. Mutational events are, therefore, closely associated with recombination in A. nidulans.
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References
Allen, M. M.: Simple conditions for the growth of unicellular blue-green algae on plates. J. Phycol. 4, 1–4 (1968).
Asato, Y., Folsome, C. E.: Temporal genetic mapping of the blue-green alga Anacystis nidulans. Genetics 65, 407–419 (1970).
Zazin, M. J.: Sexuality in a blue-green alga: genetic recombination in Anacystis nidulans. Nature (Lond.) 218, 282–283 (1968).
Craig, I. W., Leach, C. K., Carr, N. G.: Studies with deoxyribonucleic acid from blue-green algae. Arch. Mikrobiol. 65, 218–227 (1969).
Demerec, M.: “Selfers”-attributed to unequal crossovers in Salmonella. Proc. nat. Acad. Sci. (Wash.) 48, 1696–1704 (1962).
Friis, J., Flury, F., Leupold, U.: Characterization of spontaneous mutations of mitotic and meiotic origin in the ad-1 locus of Schizosaccharomyces pombe. Mutation Res. 11, 373–390 (1971).
Herdman, M.: Transformation in the blue-green alga Anacystis nidulans and an associated phenomenon of mutation. Proc. of the First European Meeting on Bacterial Transformation (in press).
Herdman, M., Carr, N. G.: Recombination in Anacystis nidulans mediated by an extracellular DNA/RNA complex. J. gen. Microbiol. 68, xiv (1971).
Herdman, M., Carr, N. G.: The isolation and characterization of mutant strains of the bluegreen alga Anacystis nidulans. J. gen. Microbiol. 70, 213–220 (1972).
Herdman, M., Faulkner, B. M., Carr, N. G.: Synchronous growth and genome replication in the blue-green alga Anacystis nidulans. Arch. Mikrobiol. 73, 238–249 (1970).
Magni, G. E.: The origin of spontaneous mutations during meiosis. Proc. nat. Acad. Sci. (Wash.) 50, 975–980 (1963).
Magni, G. E.: Spontaneous mutations. Proc. XII Int. Congress Genetics 3, 247–259 (1969).
Nester, E. W., Schafer, M., Lederberg, J.: Gene linkage in DNA transfer: a cluster of genes concerned with aromatic biosynthesis in Bacillus subtilis. Genetics 48, 529–551 (1963).
Shestakov, S. V., Khyen, N. T.: Evidence for genetic transformation in the blue-green alga Anacystis nidulans. Molec. gen. Genet. 107, 372–375 (1970).
Yoshikawa, H.: Mutations resulting from the transformation of Bacillus subtilis. Genetics 54, 1201–1214 (1966).
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Communicated by L. Lerman
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Herdman, M. Mutations arising during transformation in the blue-green alga Anacystis nidulans . Molec. Gen. Genet. 120, 369–378 (1973). https://doi.org/10.1007/BF00268150
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DOI: https://doi.org/10.1007/BF00268150