Summary
Aneuploids have been produced for the 5S RNA genes of Drosophila melanogaster by three different methods. Tandem duplications were produced at a frequency of about 0.05% by irradiation of oocytes and selection for “anti-Minutes.” Other Minute suppressors were recovered and localized to the region between nu Dand Pu 2on the genetic map. One, Su M1, reduced crossing-over as a heterozygote and is tentatively identified as a duplication. Deficiencies for the 5S RNA genes were also produced, which showed no dominant phenotype. A Minute deficiency was localized to region 56F-57A on the salivary map and was shown to be allelic to M(2) 173. The “anti-Minute” method of selection should be useful for producing tandem duplications throughout the genome.
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Communicated by E. Hadorn
Postdoctoral investigator supported by subcontract No. 3322 from the Biology Division of Oak Ridge National Laboratory to the University of Tennessee.
Operated by Union Carbide Corporation for the U.S. Atomic Energy Commission.
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Nix, C.E. Molecular studies on the 5S RNA genes of Drosophila melanogaster . Molec. Gen. Genet. 120, 309–318 (1973). https://doi.org/10.1007/BF00268145
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DOI: https://doi.org/10.1007/BF00268145