Molecular and General Genetics MGG

, Volume 146, Issue 2, pp 139–145 | Cite as

Multiple regulation of nucleoside catabolizing enzymes: Effects of a polar dra mutation on the deo enzymes

  • Hanne Albrechtsen
  • Karin Hammer-Jespersen
  • Agnete Munch-Petersen
  • Niels Fiil


Strains with an amber, polar mutation in the dra1 gene have been isolated. The mutation was introduced into a set of isogenic strains, wild type or with concurrent regulatory mutations, and further characterized by suppression and heat inactivation experiments.

The effect of the polar dra mutation on the three remaining genes of the deo operon, the tpp, drm and pup genes, was determined by estimating the enzyme levels in the various dra-mutants. The effect was found to be non-coordinate, indicating the formation in the cells of two types of transcripts: A tetracistronic unit, containing the message from all four genes, and a dicistronic unit, covering the two distal genes only.


Enzyme Nucleoside Enzyme Level Distal Gene Multiple Regulation 
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Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Hanne Albrechtsen
    • 1
  • Karin Hammer-Jespersen
    • 1
  • Agnete Munch-Petersen
    • 1
  • Niels Fiil
    • 2
  1. 1.Institute of Biological Chemistry BUniversity of CopenhagenDenmark
  2. 2.Microbiological InstituteUniversity of CopenhagenDenmark

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