Summary
Strains with an amber, polar mutation in the dra 1 gene have been isolated. The mutation was introduced into a set of isogenic strains, wild type or with concurrent regulatory mutations, and further characterized by suppression and heat inactivation experiments.
The effect of the polar dra mutation on the three remaining genes of the deo operon, the tpp, drm and pup genes, was determined by estimating the enzyme levels in the various dra-mutants. The effect was found to be non-coordinate, indicating the formation in the cells of two types of transcripts: A tetracistronic unit, containing the message from all four genes, and a dicistronic unit, covering the two distal genes only.
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References
Ahmad, S.I., Pritchard, R.H.: A map of four genes specifying enzymes involved in catabolism of nucleosides and deoxynucleosides in Escherichia coli. Molec. gen. Genet. 104, 351–356 (1969)
Ahmad, S.I., Pritchard, R.H.: A regulatory mutant affecting the synthesis of enzymes involved in the catabolism of nucleosides in Escherichia coli. Molec. gen. Genet. 111, 77–83 (1971)
Ahmad, S.I., Pritchard, R.H.: An operator constitutive mutant affecting the synthesis of two enzymes involved in the catabolism of nucleosides in Escherichia coli. Molec. gen. Genet. 124, 321–329 (1973)
Baumanis, G.E., Smirnov, Yu, V., Sukhodoletz, V.V.: Production and study of polar mutants for nucleoside catabolism linked genes in Escherichia coli. Genetika 5, 81–88 (1974)
Bonney, R.J., Weinfeld, H.: Regulation of thymidine metabolism in Escherichia coli K-12: Studies on the inducer and the coordinateness of induction of the enzymes. J. Bact. 106, 812–818 (1971)
Breitman, T.R., Bradford, R.M.: The absence of deoxyriboaldolase activity in a thymineless mutant of Escherichia coli strain 15: A possible explanation for the low thymine requirement of some thyminelless strains. Biochim. biophys. Acta (Amst.) 138, 217–220 (1967)
Buxton, R.S.: A genetic analysis of thymidine-resistant and lowthymine requiring mutants of Escherichia coli K12 induced by bacteriophage Mu-1. J. Bact. 121, 475–484 (1975)
Hammer-Jespersen, K., Munch-Petersen, A.: Phosphodeoxyribomutase from Escherichia coli: Purification and some Properties. Europ. J. Biochem. 17, 397–407 (1970)
Hammer-Jespersen, K., Munch-Petersen, A.: Multiple regulation of nucleoside catabolizing enzymes: Regulation of the deo operon by the cytR and deoR gene products. Molec. gen. Genet. 137, 327–335 (1975)
Hammer-Jespersen, K., Munch-Petersen, A., Nygaard, P., Schwartz, M.: Induction of enzymes involved in the catabolism of deocyribonucleosides and ribonucleosides in Escherichia coli K12. Euron. J. Biochem. 19, 533–538 (1971)
Hoffee, P.A.: 2-deoxyribose gene-enzyme complex in Salmonella typhimurium. I. Isolation and enzymatic characterisation of 2-deoxyribose-negative mutants. J. Bact. 95, 449 (1968a)
Karlström, O.: Mutants of Escherichia coli defective in ribonucleoside and deoxyribonucleoside catabolism. J. Bact. 95, 1069–1077 (1968)
Martin, R.G., Silbert, D.F., Smith, D.W.E., Whitfield, H.J.: Polarity in the histidine operon. J. molec. Biol. 21, 357–369 (1966)
Miller, J.H.: Experiments in molecular genetics. New York: Cold Spring Harbor Laboratory 1972
Monod, J., Cohen-Bazire, G., Cohn, M.: Sur la biosynthese de la beta-galactosidase (lactose) chez Escherichia coli. La specificité de l'induction. Biochim. biophys. Acta (Amst.) 7, 585–599 (1961)
Munch-Petersen, A., Nygaard, P., Hammer-Jespersen, K., Fiil, N.: Mutants constitutive for nucleoside-catabolizing enzymes in Escherichia coli K12. Isolation, characterisation and mapping. Europ. J. Biochem. 27, 208–215 (1972)
Svenningsen, B.: Regulated in vitro synthesis of the enzymes of the deo operon in Escherichia coli. Properties of the DNA directed system. Molec. gen. Genet. 137, 289–304 (1975)
Yanofsky, C., Ito, J.: Nonsense codons and polarity in the tryptophan operon. J. molec. Biol. 21, 313–334 (1966)
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Albrechtsen, H., Hammer-Jespersen, K., Munch-Petersen, A. et al. Multiple regulation of nucleoside catabolizing enzymes: Effects of a polar dra mutation on the deo enzymes. Molec. Gen. Genet. 146, 139–145 (1976). https://doi.org/10.1007/BF00268082
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DOI: https://doi.org/10.1007/BF00268082