Advertisement

Molecular and General Genetics MGG

, Volume 146, Issue 2, pp 133–137 | Cite as

Cytoplasmic inheritance of antimycin A resistance in Saccharomyces cerevisiae

  • Georg Michaelis
Article

Summary

Three antimycin resistant mutants of Saccharomyces cerevisiae are characterized genetically. The mutations have been shown to be cytoplasmically inherited by four criteria. The phenotype persists in diploids formed by a cross with a ρ0 strain of yeast of the opposite mating type. Diploids heterozygous for the antimycin marker, however, show segregation of the resistance and sensitivity during mitosis. Tetrad analysis indicated a non-Mendelian segregation (4:0 and 0:4) of the mutations. The antimycin marker can be eliminated by ethidium bromide treatment under conditions that should have deleted all of the mitochondrial DNA.

Keywords

Bromide Ethidium Ethidium Bromide Saccharomyces Cerevisiae Saccharomyces 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Butow, R.A., Zeydel, M.: The isolation of an antimycin-resistant mutant of Torulopsis utilis. J. biol. Chem. 243, 2545–2549 (1968)Google Scholar
  2. Coen, D., Deutsch, J., Netter, P., Petrochilo, E., Slonimski, P.P.: Mitochondrial Genetics I. Methodology and Phenomenology. Symp. Soc. exp. Biol. 24, 449–496 (1970)Google Scholar
  3. Fowell, R.R.: Sporulation and hybridization of yeasts. In: The yeasts, Vol. I (Rose A.H., Harrison, J.S., eds.), pp. 303–383. London-New York: Academic Press 1969Google Scholar
  4. Grimmelikhuijzen, C.J.P., Slater, E.C.: Antimycin-insensitive mutants of Candida utilis. I. Isolation and characterization of mutant 28. Biochim. biophys. Acta (Amst.) 305, 67–79 (1973)Google Scholar
  5. Lang, B., Burger, G., Wolf, K., Bandlow, W., Kaudewitz, F.: Studies on the mechanism of electron transport in the bc1-segment of the respiratory chain in yeast. III. Isolation and characterization of an antimycin resistant mutant ANT8 in Schizosaccharomyces pombe. Molec. gen. Genet. 137, 353–363 (1975)Google Scholar
  6. Michaelis, G., Douglass, S., Tsai, M.-J., Criddle, R.S.: Mitochondrial DNA and suppressiveness of petite mutants in Saccharomyces cerevisiae. Biochem. Genet. 5, 487–495 (1971)Google Scholar
  7. Mortimer, R.K., Hawthorne, D.C.: Yeast genetics. In: The yeast, Vol. 1 (Rose, A.H., Harrison, J.S., eds.), pp. 386–460. London-New York: Academic Press 1969Google Scholar
  8. Slonimski, P.P., Tzagoloff, A.: Localization in yeast mitochondrial DNA of mutations expressed in a deficiency of cytochrome oxidase and/or coenzyme QH2-cytochrome c reductase. Europ. J. Biochem. 61, 27–41 (1976)Google Scholar
  9. Subik, J.: Mueidin-resistant antimycin A-sensitive mitochondrial mutant of Saccharomyces cerevisiae. FEBS Letters 59, 273–276 (1975)Google Scholar
  10. Tzagoloff, A., Akai, A., Needleman, R.B.: Assembly of the mitochondrial membrane system. Characterization of nuclear mutants of Saccharomyces cerevisiae with defects in mitochondrial ATPase and respiratory enzymes. J. biol. Chem. 250, 8228–8235 (1975a)Google Scholar
  11. Tzagoloff, A., Akai, A., Needleman, R.B., Zulch, G.: Assembly of the mitochondrial membrane system. Cytoplasmic mutants of Saccharomyces cerevisiae with lesions in enzymes of the respiratory chain and in the mitochondrial ATPase. J. biol. Chem. 250, 8236–8242 (1975b)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Georg Michaelis
    • 1
  1. 1.Physiologisch-Chemisches Institut der Universität WürzburgWürzburgFederal Republic of Germany

Personalised recommendations