Summary
The timing of host-cell lysis by coliphage λ is controlled by two separable systems. The λS gene product acts as a positive effector of lysis while another protein, the lysis regulator, is an inhibitor of lysis. It the continuous function of the lysis regulator is interrupted in phage infected cells immediate lysis ensues. This lysis requires metabolic energy but not S gene activity. In contrast, S protein activity is stimulated by agents which uncouple and which block oxidative phosphorylation. The lysis regulator is thermolabile and several lines of evidence suggest that it may be the λrex gene product.
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Adhya, S., Sen, A., Mitra, S.: The role of gene S. In: The bacteriophage lambda (ed. by Hershey, A. D.), Cold Spr. Harb. Laboratory, p. 743 (1971)
Beckey, A. D., Wulff, J. L., Earhart. C. F.: Early synthesis of membrane protein after bacteriophage T4 infection. J. Virol. 14, 886 (1974)
Bernstein, A., Rolfe, B. and Onodera, K.: Pleiotrophic properties and genetic organisation of the tolA, B locus of escherichia coli K-12. J. Bact. 112, 74 (1972)
Campbell, A., Del Campillo-Campbell, A.: Mutant of bacteriophage λ producing a thermolabile endolysin. J. Bact. 85, 1202 (1963)
Christensen, J. R., Geiman, J. M.: A new effect of the rex gene of phage λ: Premature lysis after infection by phage Tl. Virology 56, 285 (1973)
Cohen, S. N., Chang, A. C. Y.: Genetic expression in bacteriophage λ III. Inhibition of Escherichia coli nucleic acid and protein synthesis during λ development. J. molec. Biol. 49, 557 (1970)
Couse, N. L.: Control of lysis of T4-infected Escherichia coli J. Virol. 2, 198 (1968)
Dove, W. F.: Strains of phage λ in current use. Virology 38, 349 (1969)
Goldberg, A. R., Howe, M.: New mutations in the S cistron of bacteriophage lambda affecting host cell lysis. Virology 38, 200 (1969)
Griffith, J., Kornberg, A.: In: Membrane research (ed. by Fox. C. F.), p. 281, New York: Academic Press 1972
Harris, A. W., Mount, D. W. A., Fuerst, C. R., Siminovitch, L.: Mutations in bacteriophage λ affecting host cell lysis. Virology 32, 553 (1967)
Kingsbury, D. T., Sieckman, D. G., Helinski, D. R.: Temperature sensitive mutants for the replication of plasmids in Escherichia coli. I. Isolation and specificity of host and plasmid mutations. Genetics 74, 1 (1973)
Luria, S. E.: Phage, colicins, and macroregulatory phenomena. Science 168, 1166 (1970)
Mark, K. K., Szybalski, W.: Repressor and rex product of coliphage lambda: Lack of collaboration and joint controls. Molec. gen. Genet. 123, 123 (1973)
Ozaki, M., Mizushima, S., Nomura, M.: Identification and functional characterization of the protein controlled by the streptomycin-resistant locus in Escherichia coli. Nature (Lond.) 222, 333 (1969)
Reader, R. W., Siminovitch, L.: Lysis defective mutants of bacteriophage lambda: genetics and physiology of S cistron mutants. Virology 43, 607 (1971a)
Reader, R. W., Siminovitch, L.: Lysis defective mutants of bacteriophage lambda: on the role of the S function in lysis. Virology 43, 623 (1971b)
Rolfe, B., Schell, J., Becker, A., Heip, J., Onodera, K., Schell-Frederick, E.: A colicin-tolerant mutant of Escherichia coli with reduced levels of cyclic AMP and a strong bias towards lysogeny. Molec. gen. Genet. 120, 1 (1973)
Rolfe, B. G., Campbell, J. H.: A relationship between tolerance to colicin K and the mechanism of phage-induced host cell lysis. Molec. gen. Genet. 133, 293 (1974)
Sussman, R., Jacob, F.: Sur un système de repression thermosensible chez le bacteriophage d'Escherichia coli. C. R. Acad. Sci. (Paris) 254, 1517 (1962)
Taylor, A.: Endopeptidase activity of phage λ-endolysin. Nature (Lond.) New Biol. 234, 144 (1971)
Tomizawa, J. I., Ogawa, T.: Inhibition of growth of rII mutants of bacteriophage T4 by the immunity substance of bacteriophage lambda. J. molec. Biol. 23, 277 (1967)
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Campbell, J.H., Rolfe, B.G. Evidence for a dual control of the initiation of host-cell lysis caused by phage lambda. Molec. Gen. Genet. 139, 1–8 (1975). https://doi.org/10.1007/BF00267990
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DOI: https://doi.org/10.1007/BF00267990