Summary
Two F- mutants deficient in conjugation with F-type donors are isolated and characterized. Phenotypically, these mutants are similar; they have heptose-less lipopolysaccharide and lack some outer membrane protein. Genotypically, they are different. One mutant harbours a point mutation in the 70 to 74 min region, while the other is deleted for the chromosomal region 6.5 to 8.5 min. Comparison of the properties of the conjugation-deficient mutants described in this paper with other such mutants suggests than an outer membrane protein is the receptor for the F-pilus.
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Communicated by R. Devoret
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Havekes, L.M., Lugtenberg, B.J.J. & Hoekstra, W.P.M. Conjugation deficient E. coli K12 F- mutants with heptose-less lipopolysaccharide. Molec. Gen. Genet. 146, 43–50 (1976). https://doi.org/10.1007/BF00267981
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DOI: https://doi.org/10.1007/BF00267981