Summary
In a mutant strain defective in polynucleotide phosphorylase, under conditions where the enzyme becomes limiting, it is possible to demonstrate that chemical as well as functional half lives of mRNA become longer if the strain is also missing ribonuclease II. These results allow to unify in a simple model a variety of observations about turnover of RNA in a variety of bacteria.
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Apirion, D.: Degradation of RNA in Escherichia coli. A hypothesis. Molec. gen. Genet. 122, 313–322 (1973)
Apirion, D., Watson, N.: Mapping and some characterization of a mutation in Escherichia coli that reduces the level of RNase III: a ribonuclease specific for double stranded RNA. J. bacteriol., in press
Blundell, M., Kennell, D.: Evidence for endonucleolytic attack in decay of lac messenger RNA in Escherichia coli. J. molec. Biol. 83, 143–154 (1974)
Chaney, S. G., Boyer, P. D.: Incorporation of water oxygens into intracellular nucleotides and RNA: II. Predominantly hydrolytic RNA turnover in Escherichia coli. J. molec. Biol. 64, 581–591 (1972)
Cohen, P. T., Yaniv, M., Yanofsky, C.: Nucleotide sequences from messenger RNA transcribed from the operator-proximal portion of the tryptophan operon of Escherichia coli. J. molec. Biol. 74, 163–172 (1973)
Duffy, J. J., Chaney, S. G., Boyer, P. D.: Incorporaton of water oxygens into intracellular nucleotides and RNA I. Predominantly non-hydrolytic RNA turnover in Bacillus subtilis. J. molec. Biol. 64, 565–579 (1972)
Fitt, P. S., See, Y. P.: Guinea-pig liver Polynucleotide phosphorylase. Biochem. J. 116, 309–311 (1970)
Grunberg-Manago, M.: Polynucleotide phosphorylase. In: Progress in Nucleic Acid Research (Eds. J. N. Davidson and W. E. Cohn. Acad. Press) 1, 93–133 (1963)
Immken, L., Apirion, D.: Site of action of 3′,5′-cyclic adenosine monophosphate in production of tryptophanase in Escherichia coli. Genetics 70, 175–180 (1972)
Kaplan, R., Apirion, D.: The involvement of ribonuclease I, ribonuclease II and polynucleotide phosphorylase in the degradation of stable RNA during carbon starvation in E. coli. J. biol. Chem. 249, 149–151 (1974)
Kaplan, R., Apirion, D.: Decay of ribosomal RNA in Escherichia coli cells starved for a carbon source. J. biol. Chem. 250, 1854–1863 (1975a)
Kaplan, R., Apirion, D.: Decay of ribosomal RNA in Escherichia coli cells starved for various nutrients. J. biol. Chem. 250, 3174–3178 (1975b)
Kimhi, Y., Littauer, U. Z.: Purification and propeties of polynucleotide phosphorylase from Escherichia coli. J. biol. Chem. 243,231–240 (1968)
Kinscherf, T. G., Lee, Y. F., Apirion, D.: A technique for the isolation of mutants of Escherichia coli affected in degradation of cellular RNA. Nucleic acid Research 1, 1439–1453 (1974)
Klee, C. B., Singer, M. F.: The processive degradation of individual polyribonucleotide chains. II. Micrococcus lysodeikticus polynucleotide phosphorylase. J. biol. Chem. 243, 923–927 (1968)
Krishna, R. V., Apirion, D.: Polynucleotide phosphorylase has a role in growth of Escherichia coli. J. Bact. 113, 1235–1239 (1973)
Krishna, R. V., Rosen, L., Apirion, D.: Increased inactivation and degradation of messenger ribonucleic acid in an Escherichia coli strain containing a thermolabile polynucleotide phosphorylase. Nature (Lond.) New Biol. 242, 18–20 (1973)
Lennette, E. T., Gorelic, L., Apirion, D.: An Escherichia coli mutant with increased messenger ribonuclease activity. Proc. nat. Acad. Sci. (Wash.) 68, 3140–3144 (1971)
See, Y. P., Fitt, P. S.: Rat liver mitochondrial polynucleotide phosphorylase. FEBS Lett. 15, 65–68 (1971)
Simon, M., Apirion, D.: Increased inactivation of messengers in Escherichia coli t8 mutant. Nature (Lond.) New Biol. 239, 79–81 (1972a)
Simon, M., Apirion, D.: A messenger RNA from the lactose operon of Escherichia coli that cannot direct the production of functional β-galactosidase in absence of exogenous adenosine 3′,5′-cyclic monophosphate. Genetics 71, 1–18 (1972b)
Thang M. N., Beltcher, B., Grunberg-Manago, M.: Phosphorolysis of tRNA multiple conformational states of tRNA in solution. Europ. J. Biochem. 19, 184–193 (1971)
Thang, M. N., Guschlbauer, W., Zachau, H. G., Grunberg-Manago, M.: Degradation of transfer ribonucleic acid by polynucleotide phosphorylase I. Mechanism of phosphorolysis and structure of tRNA. J. molec. Biol. 26, 403–421 (1967)
Weatherford, S. C., Rosen, L., Gorelic, L., Apirion, D.: Escherichia coli strains with thermolabile ribonuclease II activity. J. biol. Chem. 247, 5404–5408 (1972)
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Kinscherf, T.G., Apirion, D. Polynucleotide phosphorylase can participate in decay of mRNA in Escherichia coli in the absence of ribonuclease II. Molec. Gen. Genet. 139, 357–362 (1975). https://doi.org/10.1007/BF00267975
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DOI: https://doi.org/10.1007/BF00267975