Summary
Streptomycin-resistant and dependent mutants were isolated from Bacillus subtilis ATCC 6633. Ribosomal proteins were analyzed from six such mutants with chromatography on carboxymethyl cellulose or phosphocellulose columns. An altered specific 30s ribosomal protein, 30B, could be detected in all of these mutants. Streptomycin-independent revertants were isolated from a streptomycin-dependent strain. Some of them had an altered 30s ribosomal protein, 30A, and some others had an altered 30C protein. It was shown that from the data of partial N-terminal amino acid sequences together with amino acid compositions and mobilities on two-dimensional gel electrophoresis of these proteins that 30A, 30B and 30C proteins were homologous with S5, S12 and S4 of E. coli 30s ribosomal proteins, respectively.
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Birge, E. A., Kurland, C. G.: Altered ribosomal proteins in streptomycin-dependent Escherichia coli. Science 166, 1282 (1969)
Birge, E. A., Kurland, C. G.: Reversion of a streptomycin-dependent strain of Escherichia coli. Molec. gen. Genet. 109, 356 (1970)
Bollen, A., Davies, J., Ozaki, M., Mizushima, M.: Ribosomal protein conferring sensitivity to the antibiotic spectinomycin in Escherichia coli. Science 165, 85 (1969)
Dekio, S., Takata, R.: Genetic studies of the ribosomal proteins in Escherichia coli II. Altered 30s ribosomal protein component specific to spectinomycin resistant mutants. Molec. gen. Genet. 105, 219 (1969)
Dekio, S., Takata, R., Osawa, S., Tanaka, K., Tamaki, M.: Genetic studies of the ribosomal proteins in Escherichia coli IV. Pattern of the alteration of ribosomal protein components in mutants resistant to spectinomycin or erythromycin in different strains of Escherichia coli. Molec. gen. Genet. 107, 39 (1970)
Deusser, E., Stöffler, G., Wittmann, H. G., Apirion, D.: Ribosomal proteins XVI. Altered S4 proteins in Escherichia coli revertants from streptomycin dependence to independence. Molec. gen. Genet. 109, 298 (1973)
Edman, P., Begg, G.: A protein sequenator Europ. J. Biochem. 1, 80 (1967)
Funatsu, G., Nierhaus, K., Wittmann-Liebold, B.: Ribosomal proteins XXII. Studies on the altered protein S5 from a spectinomycin resistant mutant of Escherichia coli. J. molec. Biol. 64, 201 (1972)
Funatsu, G., Schiltz, E., Wittmann, H. G.: Ribosomal proteins XXVII. Localization of the amino acid exchange in protein S5 from two Escherichia coli mutants resistant to spectinomycin. Molec. gen. Genet. 114, 106 (1971)
Hardy, S. J. S., Kurland, C. G., Voynow, P., Mora, G.: The ribosomal proteins of Escherichia coli I. Purification of the 30s ribosomal proteins. Biochemistry (Wash.), 8, 2897 (1969)
Hasenbank, R., Guthrie, C., Stöffler, G., Wittmann, H. G., Rosen, L., Apirion, D.: Electrophoretic and immunological studies on ribosomal proteins of 100 Escherichia coli revertants from streptomycin dependence. Molec. gen. Genet. 127, 1 (1973)
Higo, K., Held, W., Kahan, L., Nomura, M.: Functional correspondence between 30s ribosomal proteins of Escherichia coli and Bacillus stearothermophilus. Proc. nat. Acad. Sci. (Wash.) 70, 944 (1973)
Higo, K., Loertscher, K.: Amino-terminal sequences of some Escherichia coli 30s ribosomal proteins and functionally corresponding Bacillus stearothermophilus ribosomal proteins. J. Bact. 118, 180 (1974)
Hindennach, I., Stöffler, G., Wittmann, H. G.: Ribosomal proteins. Isolation of the protein from 30s ribosomal subunit of Escherichia coli. Europ. J. Biochem. 23, 7, (1971)
Isono, K.: Altered 30s ribosomal proteins in streptomycin resistant, dependent and independent mutants of Bacillus stearothermophilus. Molec. gen. Genet. 133, 77 (1974)
Itoh, T., Wittmann, H. G.: Amino acid replacements in protein S5 and S12 of two Escherichia coli revertants from streptomycin dependence to independence. Molec. gen. Genet. 127, 19 (1973)
Kaltschmidt, E., Dzionara, M., Wittmann, H. G.: Ribosomal proteins XV. Amino acid compositions of isolated ribosomal proteins from 30s and 50s subunits of Escherichia coli. Molec. gen. Genet. 109, 292 (1970)
Kaltschmidt, E., Wittmann, H. G.: Ribosomal proteins VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Analyt. Biochem. 36, 401 (1970)
Kreider, G., Brownstein, B. L.: A mutation suppressing streptomycin dependence. II. An altered protein on the 30s ribosomal subunit. J. molec. Biol 61, 135 (1971)
Kreider, G., Brownstein, B. L.: Ribosomal proteins involved in the suppression of streptomycin dependence in Escherichia coli. J. Bact. 109, 780 (1972)
Metzger, H., Shapiro, M. B., Mosimann, J. E.: Assessment of compositional relatedness between proteins. Nature (Lond.) 219, 1166 (1968)
Osawa, S., Otaka, E., Itoh, T., Fukui, T.: Biosynthesis of 50s ribosomal subunit in Escherichia coli. J. molec. Biol. 46, 321 (1968)
Otaka, E., Itoh, T., Osawa, S.: Ribosomal proteins of bacterial cells: Strain-and species-specificity. J. molec. Biol. 33, 93 (1968)
Ozaki, M., Mizushima, S., Nomura, M.: Identification and functional characterization of the protein controlled by the streptomycin-resistant locus in Escherichia coli. Nature (Lond.) 222, 333 (1969)
Smith, I., Goldthwaite, C. G., Dubnau, D. D.: The genetics of ribosomes in Bacillus subtilis. Cold Spr. Harb. Symp. quant. Biol. 43, 85 (1969)
Takata, R., Dekio, S., Otaka, E., Osawa, S.: Genetic studies of the ribosomal proteins in Escherichia coli I. Mutants and strains having 30s ribosomal subunit with altered protein component. Molec. gen. Genet. 105, 113 (1969)
Wittmann-Liebold, B.: Studies on the primary structure of 20 proteins from Escherichia coli ribosomes by means of an improved protein sequenator. FEBS Lett. 36, 247 (1973)
Yaguchi, M., Matheson, A. T., Visentin, L. P.: Prokaryotic ribosomal proteins: N-terminal sequence homologies and structural correspondence of 30s ribosomal proteins from Escherichia coli and Bacillus stearothermophilus. FEBS Lett. 46 296 (1974)
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Itoh, T., Kosugi, H., Higo, K. et al. Ribosomal proteins from streptomycin-resistant and dependent mutants, and revertants from streptomycin-dependence to independence in Bacillus subtilis . Molec. Gen. Genet. 139, 293–301 (1975). https://doi.org/10.1007/BF00267969
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DOI: https://doi.org/10.1007/BF00267969