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Genetic recombination at the buff spore colour locus in Sordaria brevicollis

I. Analysis of flanking marker behaviour in crosses between buff mutants and wild type

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Summary

Asci showing aberrant segregation at the buff spore colour locus in Sordaria brevicollis were selected from crosses between buff mutants and wild type in the presence of closely-linked flanking markers. The frequency of crossing-over associated with aberrant segregations was calculated and corrected to allow for crossovers between the flanking markers incidental to the aberrant segregation. The average frequency of crossing over was found to be related to the class of aberrant ascus studied. 5+:3m and 3+:5m asci showed 16% associated marker recombination while 6+:2m and 2+:6m asci showed 27% recombination. The frequency of tritype and tetratype postmeiotic segregation asci was calculated. Only 3% tetratypes were found and this is thought to indicate a low frequency of symmetric hybrid DNA formation.

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Author notes

  1. Helen Sang

    Present address: Department of Biochemistry and Molecular Biology, Harvard University, 16, Divinity Avenue, 02138, Cambridge, Massachusetts, USA

Authors and Affiliations

  1. Botany School, University of Cambridge, Downing Street, Cambridge, England

    Helen Sang & Harold L. K. Whitehouse

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  1. Helen Sang
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  2. Harold L. K. Whitehouse
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Communicated by B.A. Bridges

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Sang, H., Whitehouse, H.L.K. Genetic recombination at the buff spore colour locus in Sordaria brevicollis . Molec. Gen. Genet. 174, 327–334 (1979). https://doi.org/10.1007/BF00267806

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  • DOI: https://doi.org/10.1007/BF00267806

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