Summary
The characteristics of recombination of several petite (rho -) mutants of S. cerevisiae that retain the ω-influenced region of the mitochondrial genome, identified by the markers cap1-r, ery1-r and tsr1, are described. The petites were derived from an ω− grande (rho +) strain and those petites which retain all three markers show recombination properties similar to those of the ω- parental strain. However, other rho - mutants that retain the cap1 and ery1 loci but have lost the tsr1 locus, which is located between cap1 and ery1, show markedly different properties of mitochondrial transmission and recombination, consistent with the presence of ω+ alleles. The association of an internal deletion between the cap1 and ery1 loci with a change in ω phenotype provides additional evidence for the location of ω between these two loci.
Although the petites deleted for the tsr1 locus exhibited the recombination properties of ω+ strains, it was not possible to transmit this characteristic to rho + recombinant cells. Experiments on the kinetics of elimination by ethidium bromide of the cap1 and eryl markers from the petites and measurements of the buoyant densities of their mtDNA species did not indicate major changes (such as selective sequence repetition) in the sequences of the mtDNAs. The possible nature of the changes in the mtDNAs of these petites is discussed in light of recent studies on the physical nature of the ω alleles.
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Atchison, B.A., Choo, K.B., Devenish, R.J., Linnane, A.W., Nagley, P.: Biogenesis of mitochondria. 53. Physical map of genetic, loci in the 21S ribosomal RNA region of mitochondrial DNA in Saccharomyces cerevisiae. Mol. Gen. Genet. 174, 307–316 (1979)
Bolotin, M., Coen, D., Deutsch, J., Dujon, B., Netter, P., Petrochilo, E., Slonimski, P.P.: La recombination des mitochondries chez Saccharomyces cerevisiae. Bull. Inst. Pasteur. 69, 213–239 (1971)
Borst, P., Bos, J.L., Grivell, L.A., Groot, G.S.P., Heyting, C., Moorman, A.F.M., Sanders, J.P.M., Talen, J.L., van Kreijl, C.F., van Ommen, G.J.B.: The physical map of yeast mitochondrial DNA anno 1977 (a review). In: Genetics and biogenesis of mitochondria (Kaudewitz, F., Schweyen, R.J., Bandlow, W., Wolf, K., eds.), pp. 213–254. Berlin: De Gruyter 1977
Bos, J.L., Heyting, C., Borst, P., Arnberg, A.C., Van Bruggen, E.F.J.: An insert in the single gene for the large ribosomal RNA in yeast mitochondrial DNA. Nature 275, 336–338 (1978)
Choo, K.B., Nagley, P., Lukins, H.B., Linnane, A.W.: Biogenesis of mitochondria. 47. Refined physical map of the mitochondrial genome of Saccharomyces cerevisiae determined by analysis of an extended library of genetically and molecularly defined petite mutants. Mol. Gen. Genet. 153, 279–288 (1977)
Devenish, R.T., English, K.T., Hall, R.M., Linnane, A.W., Lukins, H.B.: Biogenesis of mitochondria. 49. Identification and mapping of a new mitochondrial locus (trs1) which maps within the polar region of the yeast mitochondrial genome. Mol. Gen. Genet. 161, 251–259 (1978)
Dujon, B., Bolotin-Fukuhara, M., Coen, D., Deutsch, J., Netter, P., Slonimski, P.P., Weill, L.: Mitochondrial genetics. XL. Mutations at the mitochonrial locus ω affecting the recombination of mitochondrial genes in Sccharomyces cerevisiae. Mol. Gen. Genet. 143, 131–165 (1976)
Dujon, B., Michel, F.: Genetic and physical characterization of a segment of the mitochondrial DNA involved in the control of genetic recombination. In: The genetic function of mitochondrial DNA (Saccone, C., Kroon, A.M., eds.), pp. 175–184. Amsterdam: Elsevier/North Holland Biomedical Press 1976
Dujon, B., Slonimski, P.P., Weill, L.: Mitochondrial genetics. IX. A model for recombination and segregation of mitochondrial genomes in Saccharomyces cerevisiae. Genetics 78, 415–437 (1974)
Heyting, C., Sanders, J.P.M.: The physical mapping of some genetic markers in the 21S ribosomal region of the mitochondrial DNA of yeast. In: The genetic function of mitochondrial DNA (Saccone, C., Kroon, A.M. eds.), pp. 273–280. Amsterdam: Elsevier/North Holland Bioomedical Press 1976
Howell, N., Hall, R.M., Linnane, A.W., Lukins, H.B.: Biogenesis of mitochondria XXX. Genetic analyses of the polarity alleles in recombinants from genetic crosses. J. Bacteriol. 119, 1063–1068 (1974)
Jacq, C., Kujawa, C., Grandchamp, C., Netter, P.: Physical characterization of the difference between yeast mtDNA alleles 305-1 and 305-2. In: Genetics and biogenesis of mitochondria (Kaudewitz, F., Schweyen, R.J., Bandlow, W., Wolf, K., eds.), pp. 255–270 Berlin: De Gruyter, 1977
Linnane, A.W., Howell, N., Lukins, H.B.: Mitochondrial genetics. In: The biogenesis of mitochondria. Transcriptional translational and genetic aspect (Kroon, A.M., Saccone, C., eds.), pp. 193–213. New York: Academic Press 1974
Linnane, A.W., Kellerman, G.M., Lukins, H.B.: A survey of methods and a critique of the study of the biogenesis of mitochondria in yeast. In: Techniques of biochemical and biophysical morphology (Glick, D., Rosenbaum, R.M., eds.) Vol. 2, pp. 1–98. New York: John Wiley and Sons 1975
Lukins, H.B., English, K.J., Spithill, T.W., Devenish, R.J., Hall, R.M., Nagley, P., Linnane, A.W.: Biochemical characterization of a temperature conditional mutant having a mutation in the ω controlled region of the yeast mitochondrial genome and effect of deletion of the mutation on mitochondrial recombination. In: Genetics and biogenesis of mitochondria (Kaudewitz, F., Schweyen, R.J., Bandlow, W., Wolf, K., eds.), pp. 107–122. Berlin: De Gruyter 1977
Molloy, P., Linnane, A.W., Lukins, H.B.: Biogenesis of mitochondria. 37. Analysis of deletion of mitochondrial antibiotic resistance markers in petite mutants of Saccharomyces cerevisiae. J. Bacteriol. 122, 7–18 (1975)
Nagley, P., Gingold E.B., Linnane, A.W.: The purification of mitochondrial genes using petite mutants of yeast. In: The biogenesis of mitochondria: transcriptional, translational and genetic aspects (Kroon, A.M., Saccone, C. eds.), pp. 157–168. New York: Academic Press 1974
Nagley, P., Gingold, E.B., Lukins, H.B., Linnane, A.W.: Biogenesis of mitochondria. XXV. Studies of the mitochondrial genomes of petite mutants of yeast using ethidium bromide as a probe. J. Mol. Biol. 78, 335–350 (1973)
Nagley, P., Linnane, A.W.: Biogenesis of mitochondria. XXL. Sudies on the nature of the mitochondrial genome of yeast: the degenerative effects of ethidium bromide on mitochondrial genetic information in a repsiratory competent strain. J. Mol. Biol. 66, 181–193 (1972)
Nagley, P., Sriprakash, K.S., Linnane, A.W.: Structure, synthesis and genetics of yeast mitochondrial DNA. In: Advances in microbial physiology (Rose, A.H. and Tempest, D.W., eds.), pp. 157–277. London: Academic Press 1977
Netter, P., Petrochilo, E., Slonimski, P.P., Bolotin-Fukuhara, M., Coen, D., Deutsch, J., Dujon, B.: Mitochondrial genetics. VII. Allelism and mapping studies of ribosomal mutants resistant to chloramphenicol erythromycin and spiramycin in Saccaromyces cerevisiae. Genetics 78 1063–1100 (1974)
Perlman, P.S., Birky, C.W.: Mitochondrial genetics in bakers yeast: a molecular mechanism for recombinational polarity and suppressiveness. Proc. Natl. Acad. Sci. U.S.A. 71, 4612–4616 (1974)
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Devenish, R.J., Hall, R.M., Linnane, A.W. et al. Biogenesis of mitochondria. 52. Molec. Gen. Genet. 174, 297–305 (1979). https://doi.org/10.1007/BF00267803
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DOI: https://doi.org/10.1007/BF00267803