Summary
The morphology of 26 cases of osteogenic sarcoma was studied using electron microscopic techniques, and the localization of acid and alkaline phosphatase activity at the ultrastructural level elucidated. Four different cells were present in the tumours: osteoblast-like, fibroblast-like, chondroblast-like, and multinucleated giant cells. The osteoblast-like cell was present in most of the tumours studied. Acid phosphatase activity was present in lysosome-like structures of almost all the cell-types studied. Alkaline phosphatase activity was noted in or on the plasma membranes and associated vesicles of osteoblast-like, fibroblast-like, and multinucleated giant cells. The abundant reaction product deposition of alkaline phosphatase as compared with the lower acid phosphatase activity is in agreement with the nature of this bone-forming tumour. The results of the histochemical studies have added to the understanding of the pathobiology of the different cells composing osteogenic sarcomas.
Résumé
La morphologie de 26 sarcomes ostéogéniques a été étudiée par microscopie électronique et la localisation des phosphatases acide et alcaline a pu être précisée au niveau ultrastructural. Quatre différents types de cellules ont été mis en évidence dans les tumeurs: cellules d'aspect ostéoblastique, fibroblastique et chondroblastique, ainsi que des cellules géantes multinuclées. Les cellules d'aspect ostéoblastique étaient présentes dans presque toutes les tumeurs étudiées. Une activité phosphatasique acide a été trouvée dans les lysosomes de toutes les cellules étudiées. Une activité phosphatasique alcaline a été observée dans — ou sur — la membrane plasmatique et dans les vésicules associées des cellules d'aspect ostéoblastique et fibroblastique ainsi que des cellules géantes. L'abondance des produits de réaction de la phosphatase alcaline contrastant avec une faible activité phosphatasique acide est en accord avec la nature de cette tumeur ostéogénique. Les résultats de l'étude histochimique ont aidé à comprendre la pathobiologie des différentes cellules qui constituent les sarcomes ostéogéniques.
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Aparisi, T., Stark, A. & Ericsson, J.L.E. Human osteogenic sarcoma. International Orthopaedics 6, 171–179 (1982). https://doi.org/10.1007/BF00267727
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DOI: https://doi.org/10.1007/BF00267727